chimeraviz - import own fusion file for visualization
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@verenatischler-13394
Last seen 7.4 years ago

Hello,

I would like to use the package "chimeraviz" to visualize fusions.

"chimeraviz allows you to import data from nine different fusion-finders: deFuse, EricScript, InFusion, JAFFA, FusionCatcher, FusionMap, PRADA, SOAPFuse, and STAR-FUSION."

This is information from the "chimeraviz vignette" about how the fusion list is imported

# Load chimeraviz
library(chimeraviz)
# Load SOAPfuse data
if(!exists("soapfuse833ke"))
  soapfuse833ke <- system.file(
    "extdata",
    "soapfuse_833ke_final.Fusion.specific.for.genes",
    package = "chimeraviz")
fusions <- importSoapfuse(soapfuse833ke, "hg38", 10)

I would like to import my own fusion file for visualization but cannot find a way how to import my text file. I would be very grateful for suggestions. Thank you very much!

 

visualization chimeraviz • 1.8k views
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stianlagstad ▴ 90
@stianlagstad-9723
Last seen 4.7 years ago

Thank you for your interest in using chimeraviz!:) You can use your own file with the import functions like this:

# Load chimeraviz
library(chimeraviz)
# A reference to your fusion file
myfile <- "/home/stian/dev/soapfuse_833ke_final.Fusion.specific.for.genes"
fusions <- importSoapfuse(myfile, "hg38", 10)

See that I've put the path to a fusion file that I have on my disk in the variable "myfile". I use that variable in the import function.

Note that the fusion file has to be from one of the supported fusion finders and you need to use the corresponding import function:
- deFuse -> importDefuse()
- EricScript -> importEricscript()
- InFusion -> importInfusion()
- JAFFA -> importJaffa()
- FusionCatcher -> importFusioncatcher()
- FusionMap -> importFusionmap()
- PRADA -> importPrada()
- SOAPFuse -> importSoapfuse()
- STAR-FUSION -> importStarfusion()

If you have a custom format for your fusion file, then you have to write your own script to import the data into Fusion objects. This isn't very difficult. Take a look at the importDefuse script here and do something similar: https://github.com/stianlagstad/chimeraviz/blob/master/R/importDefuse.R

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@verenatischler-13394
Last seen 7.4 years ago

Thanks a lot Stian for your explanations. I will write my own script for our custom files.

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0
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No problem! Just ask more here or create an issue in the github repository if there's anything else.

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Hi to chimeraviz TEAM, thanks to your outstanding work, i have a question and i am wondering , is there any ways to import the Fusion genes which detected by SQANTI package, to chimeroviz and plot them?????

Thanks in advance

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