I'm working with the following R code where I'm returning gene ontology terms pertinent to the biological process (BP) category:
> source("https://bioconductor.org/biocLite.R") > biocLite("mygene") > xli <- c('BRCA1', 'BRCA2', 'SOX2', 'MYC') > res <- queryMany(xli, scopes='symbol', fields=c('go'), species='rat') Finished > unlist(unlist(res$go.BP)[names(unlist(res$go.BP)) == 'term'], use.names = F) [1] "double-strand break repair via homologous recombination" [2] "double-strand break repair via homologous recombination" [3] "double-strand break repair via homologous recombination" [4] "DNA replication" [5] "DNA replication" . . . [378] "positive regulation of DNA biosynthetic process" [379] "positive regulation of response to DNA damage stimulus" [380] "positive regulation of response to DNA damage stimulus" [381] "positive regulation of ATP biosynthetic process" [382] "positive regulation of apoptotic signaling pathway"
Please see: https://www.biostars.org/p/120691/. But then when I try adding gene CARNS1 to xli
:
> xli <- c('BRCA1', 'BRCA2', 'SOX2', 'MYC', 'CARNS1') > res <- queryMany(xli, scopes='symbol', fields=c('go'), species='rat') Finished > unlist(unlist(res$go.BP)[names(unlist(res$go.BP)) == 'term'], use.names = F) Error in rbind(deparse.level, ...) : numbers of columns of arguments do not match
Anyone know why this error happens? At first, I thought it was because of the scopes
argument in queryMany()
, but playing around with the parameters of http://mygene.info/doc/query_service.html#available-fields did not help clarify the issue.
Any suggestions would be appreciated.
P.S. I would also welcome suggestions and/or minimal working (code) examples for using other R packages to get the job done. In fact, just to emphasize this point, if the above seems to be an intractable problem (for whatever reason), I completely do not mind using an alternative software package.
P.P.S. My sessionInfo()
is:
> sessionInfo() R version 3.3.3 (2017-03-06) Platform: x86_64-apple-darwin13.4.0 (64-bit) Running under: OS X Yosemite 10.10.5 locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages: [1] splines stats4 parallel stats graphics grDevices utils datasets methods base other attached packages: [1] geneXtendeR_1.0.2 data.table_1.10.4 mygene_1.8.0 [4] GenomicFeatures_1.24.5 AnnotationDbi_1.34.4 DEGseq_1.26.0 [7] samr_2.0 matrixStats_0.52.2 impute_1.46.0 [10] qvalue_2.4.2 PoissonSeq_1.1.2 combinat_0.0-8 [13] BiocInstaller_1.22.3 baySeq_2.6.0 perm_1.0-0.0 [16] abind_1.4-5 EBSeq_1.12.0 testthat_1.0.2 [19] blockmodeling_0.1.9 DESeq2_1.12.4 SummarizedExperiment_1.2.3 [22] Biobase_2.32.0 edgeR_3.14.0 limma_3.28.21 [25] dplyr_0.7.1 gplots_3.0.1 shinyapps_0.4.1.8 [28] shiny_1.0.3 bindrcpp_0.2 rtracklayer_1.32.2 [31] GenomicRanges_1.24.3 GenomeInfoDb_1.8.7 IRanges_2.6.1 [34] S4Vectors_0.10.3 BiocGenerics_0.18.0 devtools_1.13.2 loaded via a namespace (and not attached): [1] backports_1.1.0 Hmisc_4.0-3 plyr_1.8.4 lazyeval_0.2.0 [5] BiocParallel_1.6.6 ggplot2_2.2.1 digest_0.6.12 htmltools_0.3.6 [9] gdata_2.18.0 magrittr_1.5 checkmate_1.8.3 memoise_1.1.0 [13] cluster_2.0.6 Biostrings_2.40.2 annotate_1.50.1 colorspace_1.3-2 [17] blob_1.1.0 crayon_1.3.2 RCurl_1.95-4.8 jsonlite_1.5 [21] roxygen2_6.0.1 genefilter_1.54.2 bindr_0.1 survival_2.41-3 [25] glue_1.1.1 gtable_0.2.0 zlibbioc_1.18.0 XVector_0.12.1 [29] scales_0.4.1 DBI_0.7 Rcpp_0.12.11 xtable_1.8-2 [33] htmlTable_1.9 foreign_0.8-69 bit_1.1-12 Formula_1.2-2 [37] sqldf_0.4-11 htmlwidgets_0.9 httr_1.2.1 RColorBrewer_1.1-2 [41] acepack_1.4.1 pkgconfig_2.0.1 XML_3.98-1.9 nnet_7.3-12 [45] locfit_1.5-9.1 rlang_0.1.1 reshape2_1.4.2 munsell_0.4.3 [49] tools_3.3.3 gsubfn_0.6-6 RSQLite_2.0 stringr_1.2.0 [53] knitr_1.16 bit64_0.9-7 caTools_1.17.1 mime_0.5 [57] xml2_1.1.1 biomaRt_2.28.0 curl_2.7 tibble_1.3.3 [61] geneplotter_1.50.0 stringi_1.1.5 desc_1.1.0 lattice_0.20-35 [65] Matrix_1.2-10 commonmark_1.2 bitops_1.0-6 httpuv_1.3.5 [69] R6_2.2.2 latticeExtra_0.6-28 KernSmooth_2.23-15 gridExtra_2.2.1 [73] gtools_3.5.0 assertthat_0.2.0.9000 chron_2.3-50 proto_1.0.0 [77] rprojroot_1.2 withr_1.0.2 GenomicAlignments_1.8.4 Rsamtools_1.24.0 [81] grid_3.3.3 rpart_4.1-11 git2r_0.18.0 base64enc_0.1-3