We work on the legume Chickpea which genome has been recently released but it does not have a BSgenome package so far. I though I'd try and make one.
Following the vignette I have placed some FASTA chromosomes in a folder. I have started with a small example, so I have used just the first two chromosomes. I have two FASTA files named “Ca_chr1.fa” and “Ca_chr2.fa” located in
/Users/Cicer/seqs/
Then I have created a seed file ("cicer") which it is also included into that folder. The seed file looks like this :
Package: BSgenome.Carietinum.NCBI.ca1
Title: Cicer arietinum (Chickpea) full genome (NCBI version ASM33114v1)
Description: Cicer arietinum (Chickpea) full genome as provided by NCBI (ASM33114v1, Jan. 2013) and stored in Biostrings objects.
Version: 1.0
organism: Cicer arietinum
species: Chickpea
provider: NCBI
provider_version: ASM33114v1
release_date: Jan. 2013
release_name: BGI-Shenzhen ASM33114v1
source_url: https://www.ncbi.nlm.nih.gov/assembly/GCF_000331145.1/#/def
organism_biocview: Cicer_arietinum
BSgenomeObjname: Carietinum
seqnames: paste("Ca_", paste("chr", c(1:2), sep=""), sep="")
seqs_srcdir: /Users/Cicer/seqs/
After running `forgeBSgenomeDataPkg("path/to/cicer")`
i get this error:
Error in makeS4FromList("BSgenomeDataPkgSeed", x) :
some names in 'x' are not valid BSgenomeDataPkgSeed slots (species)
In addition: Warning message:
In readLines(infile, n = 25000L) :
incomplete final line found on './seqs/cicer'
Any suggestion it is greatly appreciated. Thanks.
> sessionInfo()
R version 3.2.3 (2015-12-10)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.11.4 (El Capitan)
locale:
[1] C
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets methods
[9] base
other attached packages:
[1] BSgenome_1.38.0 rtracklayer_1.30.4 Biostrings_2.38.4 XVector_0.10.0
[5] GenomicRanges_1.22.4 GenomeInfoDb_1.6.3 IRanges_2.4.8 S4Vectors_0.8.11
[9] BiocGenerics_0.16.1
loaded via a namespace (and not attached):
[1] XML_3.98-1.5 Rsamtools_1.22.0 GenomicAlignments_1.6.3
[4] bitops_1.0-6 futile.options_1.0.0 zlibbioc_1.16.0
[7] futile.logger_1.4.3 lambda.r_1.1.9 BiocParallel_1.4.3
[10] tools_3.2.3 Biobase_2.30.0 RCurl_1.95-4.8
[13] SummarizedExperiment_1.0.2
@Jim: the OP is using an old BioC (3.2). Maybe that's why s/he's putting species in his/her seed file.
@jodiera: We don't support old BioC versions. Please update to the current version (BioC 3.4, requires R 3.3). The current version of the BSgenome package is 1.42.0 and the BSgenomeForge vignette in it has been updated since BSgenome 1.38.0.
H.
@Hervé Well maybe, but doesn't 2015-03-26 precede BioC 3.1? By my count the last time species was a valid name was in BioC 3.0...
------------------------------------------------------------------------
r101235 | hpages@fhcrc.org | 2015-03-26 13:33:49 -0700 (Thu, 26 Mar 2015) | 3 lines
Replace 'species' field with 'common_name' in BSgenome data package seed and
DESCRIPTION files.
You're right. I just noticed the OP was using an old BioC and remembered that species went away at some point, but was too lazy to find out exactly when that change was made. So s/he might actually have been looking at an even older version of the BSgenomeForge vignette.
Thanks for helping. Thumb up from me and I hope the OP will do the same :-)