I have a question regarding one of the experiment that we just got rna seq data for, let me first show the design it looks like this,
We do not have replicates for any model (I hope we can still get some data from this model)
we would like to compare changes in gene expression and junction/exon expression between each time point to the vehicle
i.e (veh vs 30min, veh vs 1 hour, beh vs 2 hours, veh vs 3 hours)
My question is what should the design model look like for deseq2 and dexseq and what are the steps that we need to consider before we do this analysis, would not having replicates be a big issue?
Apologies for asking help upfront, we do not have a statistician that we can reach out on our facility.