Question

ddCT Package for StepOnePlus qPCR output

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rbronste ▴ 60

@rbronste-12189

Last seen 5.1 years ago

Hi,

Trying to figure out a good way to read in raw data off a StepOnePlus machine into the ddCt package? Or if any other solid suggestions for ddCt-type packages with graphical outputs. Thank you.

Rob

qPCR ddCt geneexpression • 2.1k views

ADD COMMENT • link updated 7.4 years ago by Zhang, Jitao David ▴ 120 • written 7.4 years ago by rbronste ▴ 60

score 1 · Answer 1 · 2017-07-27

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Zhang, Jitao David ▴ 120

@zhang-jitao-david-5737

Last seen 4.8 years ago

Switzerland

Dear Rob, do you mind providing an example? Thanks,

David

ADD COMMENT • link 7.4 years ago Zhang, Jitao David ▴ 120

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Hi David, thank you for taking a look, an example is linked below.

https://u105647823.dl.dropboxusercontent.com/u/105647823/ag_070617_data.xls

ADD REPLY • link 7.4 years ago rbronste ▴ 60

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Hi David, wondering if you had any thoughts on my example? Thanks again!

ADD REPLY • link 7.4 years ago rbronste ▴ 60

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Dear rbronste, not yet. I will try to catch up next week. Best wishes, David On Wed, Aug 2, 2017 at 5:57 PM, rbronste [bioc] <noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User rbronste <https: support.bioconductor.org="" u="" 12189=""/> wrote Comment: > ddCT Package for StepOnePlus qPCR output > <https: support.bioconductor.org="" p="" 98542="" #98764="">: > > Hi David, wondering if you had any thoughts on my example? Thanks again! > > ------------------------------ > > Post tags: qPCR, ddCt, geneexpression > > You may reply via email or visit https://support.bioconductor. > org/p/98542/#98764 > -- *Dr. Jitao David Zhang | 张继涛 | Computational Biology and Bioinformatics* *Roche Pharmaceutical Research and Early Development (pRED) | Pharmaceutical Sciences, Translational Technologies and Bioinformatics (PS-TTB) | Roche Innovation Center Basel | F. Hoffmann-La-Roche AG | CH-4070 Basel | Switzerland* *Building 93/3.34* *Tel +41 61 688 62 51* Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. *Please inform me immediately in case attached documents are missing!*

ADD REPLY • link 7.4 years ago Zhang, Jitao David ▴ 120