For my exploratory analysis I used the vst function to normalise my data. But I read that it is not necessary for the Differential Expression via DESeq2 since it has already a build-in normalisation on the data, is this correct? I read it in this article I believe: S. A. Michael I. Love 2019:RNA-seq workflow: gene-level exploratory analysis and differential expression
Also I understood that for the statistical analysis the raw counts and not the normalised values should be used?
Thank you, Bine