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Showing :
Normalization
•
reset
0
votes
3
replies
331
views
Combining two bulk RNA seq datasets
RNASeqData
DESeq2
Normalization
5 weeks ago
Tanvi
• 0
2
votes
2
replies
318
views
Control group between 2 batches have different expression in RNAseq data
BatchQC
BatchEffect
rnaseqGene
Normalization
updated 7 weeks ago by
jessica.mcclintock
• 0 • written 9 weeks ago by
Đình Vinh
• 0
0
votes
2
replies
300
views
Normalisation using clinical traits in RNA seq using DESeq2
DESeq2
clinicaltraits
Normalization
batchnormalisation
9 weeks ago • updated 8 weeks ago
sukeshinik5
• 0
0
votes
1
reply
218
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 10 weeks ago by
Michael Love
42k • written 10 weeks ago by
piemmea
• 0
0
votes
1
reply
213
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 11 weeks ago by
ATpoint
★ 4.2k • written 11 weeks ago by
sajadahmad41454
• 0
2
votes
2
replies
556
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind
Normalization
3 months ago
Henry
▴ 10
1
vote
6
replies
610
views
DESeq2 step-by-step Normalization then DGE separately & Normalization methods and switchability
DESeq2
Normalization
updated 3 months ago by
Michael Love
42k • written 3 months ago by
RL
• 0
0
votes
2
replies
303
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
3 months ago
Marianna
▴ 20
1
vote
4
replies
426
views
EDAseq to normalise for both gene-length and GC-content
norma
EDASeq
EDA
Normalization
4 months ago
samuel.channon
• 0
0
votes
0
replies
234
views
TCGAbiolinks STAD project 450k Methylation Beta Value data
MethylationArray
normalize450K
TCGAbiolinks
Normalization
MethylationArrayData
4 months ago
Giovanni Calice
▴ 160
2
votes
1
reply
391
views
Normalization factors when there is a possible unidirectional expression
limma
Normalization
DifferentialExpression
updated 5 months ago by
ATpoint
★ 4.2k • written 5 months ago by
Shaimaa Gamal
• 0
2
votes
2
replies
636
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
5 months ago
jackafiore
• 0
2
votes
3
replies
479
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 5 months ago by
James W. MacDonald
66k • written 5 months ago by
Shaimaa Gamal
• 0
1
vote
3
replies
747
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 6 months ago by
georgii.vdovin
• 0
0
votes
1
reply
489
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 6 months ago by
ATpoint
★ 4.2k • written 6 months ago by
Ömür Koray
• 0
2
votes
4
replies
962
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
6 months ago
Sabiha
▴ 20
1
vote
1
reply
563
views
DESeq2 Normalization for QC Samples
DESeq2
Normalization
updated 6 months ago by
Michael Love
42k • written 6 months ago by
phmai1148
• 0
2
votes
2
replies
1.1k
views
DESeq2 median of ratios normalization instead of VST or rlog for ordinations?
DESeq2
medianofratiostransformation
StatisticalMethod
Normalization
dispersion
6 months ago
Linton
• 0
0
votes
0
replies
336
views
SomaticSignatures normalization for trinucleotide motifs in reference genome
Normalization
SomaticSignatures
8 months ago
vasily.dobrovolsky
• 0
0
votes
6
replies
1.1k
views
DESeq2 Normalization with 4 Groups
Normalization
RNASeq
DESeq2
9 months ago
erika.maldonado-rosado
• 0
0
votes
0
replies
360
views
Dual species RNA-seq, Library size and normalization
Normalization
edgeR
RNASeq
DESeq2
9 months ago
Florian
• 0
1
vote
11
replies
1.3k
views
DESeq2 gene length normalisation
Normalization
DESeq2
updated 9 months ago by
Michael Love
42k • written 9 months ago by
cm15245
• 0
1
vote
4
replies
3.1k
views
How to process Nimblegen microarray .pair files to get normalized expression matrix
Microarray
Preprocessing
NimbleGen
Normalization
written 20 months ago by
Sarah
• 0
1
vote
5
replies
1.2k
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI
normalization
limma
voom
Normalization
updated 12 months ago by
Gordon Smyth
51k • written 13 months ago by
manuelsokolov
• 0
0
votes
3
replies
704
views
Normalization of different input materials
Normalization
DESeq2
updated 13 months ago by
ATpoint
★ 4.2k • written 13 months ago by
altuda
▴ 10
0
votes
2
replies
902
views
Can VST-transformed community composition data be expressed as relative abundance?
vst
relativeabundance
Normalization
DESeq2
14 months ago
dwas
• 0
5
votes
11
replies
3.8k
views
Visualization of negative log CPM values
Visualization
Normalization
ggplot2
edgeR
written 14 months ago by
Sabiha
▴ 20
0
votes
3
replies
827
views
Is the gene-specific PCR efficiency a serious concern for intrasample comparisons in RNA-Seq?
RNA-Seq
normalization
PCR_efficiency
bias
intrasample_comparison
updated 14 months ago by
James W. MacDonald
66k • written 14 months ago by
FedeXander
• 0
1
vote
2
replies
1.0k
views
Filter by expression and keep probes in limma
R
filter
limma
Normalization
Microarray
15 months ago
Abdul
▴ 20
2
votes
2
replies
745
views
Question about transforming log expression values to antilog values in limma
IlluminaChip
limma
AffymetrixChip
Normalization
Microarray
15 months ago
Abdul
▴ 20
1
vote
2
replies
680
views
RNA-Seq normalization of pre and post treatment groups
Normalization
RNASeq
written 16 months ago by
Sarah
• 0
0
votes
2
replies
875
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran
Clustering
SingleCell
Normalization
16 months ago
Assa Yeroslaviz
★ 1.5k
1
vote
6
replies
2.0k
views
can negative value microarray matrix data analysed by limma directly
limma
microarray
normalization
updated 11 months ago by
Gordon Smyth
51k • written 3.9 years ago by
linouhao
▴ 20
2
votes
3
replies
2.2k
views
deseq2 normalization and statistical method
normalization
deseq2
StatisticalMethod
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
SH
• 0
1
vote
1
reply
1.2k
views
deseq2 RLE normalization
RLE
normalization
deseq2
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
SH
• 0
0
votes
1
reply
643
views
Using DESeq2 only for normalization
DESeq2
Normalization
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
phmai1148
• 0
0
votes
1
reply
468
views
processing samples with significantly different counts
DESeq2
Normalization
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
Anastasiia
• 0
0
votes
5
replies
1.4k
views
DESeq Normalization and miRNAs Filtering
DESeq2
Normalization
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
phmai1148
• 0
2
votes
3
replies
1.1k
views
Batch correction and Deseq2
DESeq2
WGCNA
Normalization
NormalyzerDE
BatchEffect
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
suphamon.j
▴ 20
0
votes
1
reply
646
views
Normalization of RNA seq data before DESeq2 and PCA in case of strong batch effects
DESeq2
Normalization
sva
updated 16 months ago by
ATpoint
★ 4.2k • written 16 months ago by
marslena
• 0
3
votes
3
replies
894
views
What is cpmByGroup used for?
edgeR
Normalization
updated 17 months ago by
James W. MacDonald
66k • written 17 months ago by
EM
• 0
0
votes
2
replies
820
views
Using Multi-Dimensional Scaling (MDS) to produce a vector in order to account for patient bias when constructing DGE lists from RNA-seq datasets in R?
PrincipalComponent
StatisticalMethod
edgeR
MultidimensionalScaling
Normalization
17 months ago
rcreswell01
• 0
1
vote
1
reply
883
views
Library sizes of miRNA sequencing data and TMM normalization with Edge-R
edgeR
SequencingData
miRNA
TMM
Normalization
updated 17 months ago by
Gordon Smyth
51k • written 17 months ago by
tleona3
• 0
4
votes
2
replies
2.1k
views
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
DESeq2
SpikeIn
Normalization
DifferentialExpression
updated 17 months ago by
ATpoint
★ 4.2k • written 17 months ago by
kalavattam
▴ 10
0
votes
1
reply
705
views
Micro Array GEO data analysis
GEOquery
MicroarrayData
Normalization
Microarray
NanoStringDiff
updated 18 months ago by
James W. MacDonald
66k • written 18 months ago by
Aleksandra
• 0
0
votes
2
replies
1.8k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 11 weeks ago by
maria.soler
• 0 • written 18 months ago by
kalavattam
▴ 10
4
votes
8
replies
2.5k
views
How to normalize long-read RNA-seq data for comparison with short-reads
NanoporeRNASeq
LongRead
Normalization
ShortRead
IlluminaRNASeq
updated 8 days ago by
callumjcparr
• 0 • written 18 months ago by
Bernardo
▴ 10
1
vote
0
replies
649
views
Problem with VSN: Error in vsnML(sv) : L-BFGS-B needs finite values of 'fn'
justvsn
vsn
Normalization
vsn2
Proteomics
18 months ago
Arend
▴ 10
0
votes
0
replies
852
views
Trying to use a NanoString expression set with batch effects to do GSVA
GSVA
Normalization
BatchEffect
GSVAdata
NanoString
18 months ago
Zhijie
• 0
1
vote
0
replies
782
views
Use scran's normalization by deconvolution for each individual sample or over all samples
scran
Normalization
scRNA
BatchEffect
19 months ago
r_grau
▴ 10
50 results • Page
1 of 1
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Replies
Comment: 'path' must be string
by
James W. MacDonald
66k
Yes. We already knew that from the error message, and I told you that in my previous email. The point wasn't to figure out *where* the err…
Comment: 'path' must be string
by
cpierce2
• 0
I ran the debugger and this is the line that causes the error: ``` if (any(startsWith(pkcData[["RTS_ID"]][1L], "RTS")) & any(startsWith(pr…
Comment: Generating surrogate variables across multiple variables of interest
by
Robert
• 0
Yes, we have several biomarkers, and a proteomic set, so we are characterizing each biomarker by it's proteomic association(s). The use for…
Comment: Error running tximeta
by
Guido Hooiveld
★ 4.0k
FYI (since I just finalized a run with `salmon`). For each sample (`.`) an output folder is created that contains 3 files (`cmd_info.json`…
Comment: Error running tximeta
by
Michael Love
42k
Tximeta uses the full directory. There are files that give the key information about the transcriptome. It's not a good idea to selectively…
Votes
Question: Deconvolution Methods on RNA-Seq Data (Mixed cell types)
Answer: Understanding how to contrast two treatments in DESeq2
Answer: Understanding how to contrast two treatments in DESeq2
Cannot extract TxFeatures for a defined GRanges object
Limma time series spline help
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42k
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