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burcu.atasu
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@burcuatasu-14358
Last seen 6.6 years ago
Hi!
I've been performing RNAseq analysis by using svaseq package for batch effect correction. I have identified two slightly correlated known batch effect variables (such as; flowcell, date of data prep.). My question is if I use those two correlated (r=0.81) known batch effect varaibles in my models, I would overcorrect the data, or since they are slightly correlated I should use both of them and sva would take this into consideration.
Thank you in advance.
Best,
Burcu