I am running offTargetAnalysis to find gRNAs and their off-targets in some sequences. For a couple of sequences, I got the following error message while the program was trying to find all hits in a particular sequence:
"Error in .seqlengths_TwoBitFile(x) : UCSC library operation failed
Calls: offTargetAnalysis ... seqinfo -> seqinfo -> .seqlengths_TwoBitFile -> .Call
In addition: Warning message:
In .seqlengths_TwoBitFile(x) : End of file reading 4 bytes"
Could you please tell me what the cause of this error message may be?