Hi, 

I am dealing with a dataset and I do not believe any spike in were included. I want to use BASiCS to help my clustering but it requires the use of spike information. Is there a way to use BASiCs without the information?

Alternatively, is there another package I could use to use bayesian statistics on my data? 

Hello,

Indeed, the current release of BASiCS (BioC 3.6) cannot be used without spike-ins. Recently, we extended the method to the no-spikes case and that is available in BioC devel (see also https://www.biorxiv.org/content/early/2017/12/21/237214). 

However, I am afraid that BASiCS is *not* suitable for clustering analyses. This is because BASiCS has been designed in the context of supervised experimental designs only (e.g. case/control). Applying BASiCS in a clustering context is not advised and therefore I would refer you to other libraries that are specifically designed for clustering (e.g. SC3). 

My apologies for not being more helpful this time.

Best

Cata