SPIA function error in ToPASeq
0
0
Entering edit mode
fawazfebin ▴ 60
@fawazfebin-14053
Last seen 3.8 years ago

Hi

I was doing a pathway analysis of RNA-Seq data using ToPASeq program. I ran the following commands :

> library("ToPASeq")
> library("gageData")
> DEXcounts = read.table("/home/febin/DEXcounts.txt", header = TRUE ,row.names = 1)
> DEXcounts = DEXcounts[,6:ncol(DEXcounts)]
> DEXcounts <- DEXcounts[rowSums(DEXcounts)>0,]
> grpTopDex = c(rep("cntrl",2),rep("dex2",2),rep("dex4",2))
> kegg<-pathways("hsapiens","kegg")
> spi<-SPIA(DEXcounts, grpTopDex, kegg,type="RNASeq")
    Error in .local(object, which, ...) :  unused argument (stringsAsFactors = FALSE)

 

Here is my  sessionInfo

> sessionInfo()
R version 3.4.3 (2017-11-30)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 16.04.4 LTS

Matrix products: default
BLAS: /usr/lib/libblas/libblas.so.3.6.0
LAPACK: /usr/lib/lapack/liblapack.so.3.6.0

locale:
[1] LC_CTYPE=en_IN.UTF-8       LC_NUMERIC=C               LC_TIME=en_IN.UTF-8      
[4] LC_COLLATE=en_IN.UTF-8     LC_MONETARY=en_IN.UTF-8    LC_MESSAGES=en_IN.UTF-8  
[7] LC_PAPER=en_IN.UTF-8       LC_NAME=C                  LC_ADDRESS=C             
[10] LC_TELEPHONE=C             LC_MEASUREMENT=en_IN.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] grid      parallel  stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] gageData_2.16.0     ToPASeq_1.11.0      Rgraphviz_2.22.0    locfit_1.5-9.1      graph_1.56.0      
[6] BiocGenerics_0.24.0 gRbase_1.8-3        graphite_1.24.1     edgeR_3.20.9        limma_3.34.9       

loaded via a namespace (and not attached):
  [1] bitops_1.0-6               matrixStats_0.53.1         bit64_0.9-7              
  [4] progress_1.1.2             doParallel_1.0.11          RColorBrewer_1.1-2       
  [7] httr_1.3.1                 GenomeInfoDb_1.14.0        tools_3.4.3              
[10] backports_1.1.2            R6_2.2.2                   rpart_4.1-13             
[13] Hmisc_4.1-1                DBI_0.8                    lazyeval_0.2.1           
[16] colorspace_1.3-2           nnet_7.3-12                prettyunits_1.0.2        
[19] RMySQL_0.10.14             gridExtra_2.3              DESeq2_1.18.1            
[22] bit_1.1-12                 compiler_3.4.3             clipper_1.18.0           
[25] Biobase_2.38.0             htmlTable_1.11.2           DelayedArray_0.4.1       
[28] rtracklayer_1.38.3         KEGGgraph_1.38.0           scales_0.5.0             
[31] checkmate_1.8.5            mvtnorm_1.0-7              qpgraph_2.12.0           
[34] genefilter_1.60.0          RBGL_1.54.0                rappdirs_0.3.1           
[37] DESeq_1.30.0               Rsamtools_1.30.0           stringr_1.3.0            
[40] digest_0.6.15              foreign_0.8-69             R.utils_2.6.0            
[43] XVector_0.18.0             base64enc_0.1-3            pkgconfig_2.0.1          
[46] htmltools_0.3.6            maps_3.2.0                 htmlwidgets_1.0          
[49] rlang_0.2.0                rstudioapi_0.7             RSQLite_2.0              
[52] BiocParallel_1.12.0        acepack_1.4.1              R.oo_1.21.0              
[55] RCurl_1.95-4.10            magrittr_1.5               qtl_1.42-8               
[58] GenomeInfoDbData_1.0.0     Formula_1.2-2              dotCall64_0.9-5.2        
[61] Matrix_1.2-11              Rcpp_0.12.16               munsell_0.4.3            
[64] S4Vectors_0.16.0           R.methodsS3_1.7.1          stringi_1.1.7            
[67] yaml_2.1.18                SummarizedExperiment_1.8.1 zlibbioc_1.24.0          
[70] plyr_1.8.4                 blob_1.1.1                 lattice_0.20-35          
[73] Biostrings_2.46.0          splines_3.4.3              GenomicFeatures_1.30.3   
[76] annotate_1.56.2            knitr_1.20                 pillar_1.2.1             
[79] igraph_1.2.1               GenomicRanges_1.30.3       corpcor_1.6.9            
[82] biomaRt_2.34.2             codetools_0.2-15           geneplotter_1.56.0       
[85] stats4_3.4.3               XML_3.98-1.10              latticeExtra_0.6-28      
[88] data.table_1.10.4-3        spam_2.1-3                 foreach_1.4.4            
[91] gtable_0.2.0               assertthat_0.2.0           ggplot2_2.2.1            
[94] TeachingDemos_2.10         xtable_1.8-2               survival_2.41-3          
[97] tibble_1.4.2               iterators_1.0.9            GenomicAlignments_1.14.1 
[100] AnnotationDbi_1.40.0       memoise_1.1.0              IRanges_2.12.0           
[103] fields_9.6                 cluster_2.0.6      

 

How can I overcome this error? Thanks in advance.

 

ToPASeq SPIA pathway analysis R • 922 views
ADD COMMENT

Login before adding your answer.

Traffic: 1063 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6