how to run GO enrichment analysis for non-model organisms (errors when using hyperGTest)
0
0
Entering edit mode
@zhaoran1124-15562
Last seen 6.0 years ago

I am trying to run the GO enrichment analysis for a subset of genes from my non-model organism. And my gene IDs are look like :"gene1036:10"

After creating a GOHyperGParams Object using the function GSEAGOHyperGParams, I run the hyperGTest function: 

params <- GSEAGOHyperGParams(name="test",
                             geneSetCollection=mygsc, 
                             geneIds = sub_genes, 
                             universeGeneIds = genes, 
                             ontology = "MF", 
                             pvalueCutoff = 0.05, 
                             conditional = FALSE,
                             testDirection = "over")

hyperGTest(params)

Then the error occurred:

Error in getUniverseHelper(probes, datPkg, entrezIds) : 
  After filtering, there are no valid IDs that can be used as the Gene universe.
  Check input values to confirm they are the same type as the central ID used by your annotation package.
  For chip packages, this will still mean the central GENE identifier used by the package (NOT the probe IDs).

 

I used my own GO annotation file, which is just a table with gene IDs and GO IDs. Do anyone know some tools for non-model organisms? Is it possible to run enrichment analysis based on a simple GO annotation table?

 

gostats GO non_model • 1.8k views
ADD COMMENT
0
Entering edit mode

are your genes in mygsc in the same fashion as in genes? How do you  map from your ids of genes to gene ontology terms ?

ADD REPLY

Login before adding your answer.

Traffic: 938 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6