Question

steps to get up and down regulated genes using DESeq2

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Tony • 0

@tony-15866

Last seen 6.3 years ago

Which are the steps to get significantly up regulated and down regulated genes from samples which are not replicates?

By doing this step "dds <- DESeq(dds)" I am getting a warning message "same number of samples and coefficients to fit..."

Is it possible to get different combination results? The input will be multiple samples read count in a single file.

deseq2 without replicate no replicates • 3.2k views

ADD COMMENT • link updated 6.3 years ago by Michael Love 42k • written 6.3 years ago by Tony • 0

score 0 · Answer 1 · 2018-05-21

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Michael Love 42k

@mikelove

Last seen 1 day ago

United States

Replicates are required to test for significance of up and down DE.

ADD COMMENT • link 6.3 years ago Michael Love 42k

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Okay. Which are the steps we have to use to get up regulated and down regulated genes?

ADD REPLY • link 6.3 years ago Tony • 0

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Please take a look at our workflow:

http://master.bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html

ADD REPLY • link 6.3 years ago Michael Love 42k

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Is there any difference between old DESeq and DESeq2 in calculating the up and down regulated genes?

ADD REPLY • link 6.3 years ago Tony • 0

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This is answered in our papers and specifically in the vignette, see the Table of Contents. Please take some time to read over our documentation before posting. I want to focus my time on support questions not covered in the publications and documentation.

ADD REPLY • link 6.3 years ago Michael Love 42k