Problem in running DESeq2 in r
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@nazaninhoseinkhan-7443
Last seen 6.4 years ago
Iran, Islamic Republic Of

Dear all,

I am trying to run DESeq2 in R 3.3.1.

When I call library DESeq2 I get this:

"

Loading required package: GenomicRanges
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:parallel’:

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from ‘package:stats’:

    IQR, mad, xtabs

The following objects are masked from ‘package:base’:

    anyDuplicated, append, as.data.frame, cbind, colnames, do.call,
    duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect,
    is.unsorted, lapply, lengths, Map, mapply, match, mget, order,
    paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind,
    Reduce, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Loading required package: S4Vectors
Loading required package: stats4

Attaching package: ‘S4Vectors’

The following objects are masked from ‘package:base’:

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: Rcpp
Loading required package: RcppArmadillo
Warning messages:
1: multiple methods tables found for ‘fileName’ 
2: multiple methods tables found for ‘organism’"

and when I use " DESeqDataSetFromMatrix" function, I get this error message:

"Error in slot(value, what) : 
  no slot of name "exptData" for this object of class "SummarizedExperiment
""

Can u guide me how to solve this problem?

Regards

Nazanin

deseq2 DESeqDataSetFromMatrix software error • 1.2k views
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@mikelove
Last seen 1 day ago
United States

Usually this is a package version problem. Can you run:

library(BiocInstaller)

biocValid()

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0
Entering edit mode

Hi Michael,

Yes you are right. The following is what I get when I run  library(BiocInstaller) or biocValid():

downgrade with biocLite(c("AnnotationDbi", "BiocGenerics", "BiocParallel", "Biostrings",
  "bumphunter", "ChAMP", "DESeq2", "fastseg", "GenomicRanges", "genoset",
  "Gviz", "IRanges", "methyAnalysis", "minfi", "pcaMethods", "qvalue",
  "Rsamtools", "XVector"))

Error: 196 package(s) out of date; 18 package(s) too new

Now how can I solve this issue?

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Entering edit mode

Only install with biocLite() and you (should) never have this problem. Try running biocLite() and if this doesn’t work perhaps remove.package() the too new ones.

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Thank you so much for your help. It works!

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