Problem in running DESeq2 in r
1
0
Entering edit mode
@nazaninhoseinkhan-7443
Last seen 5.9 years ago
Iran, Islamic Republic Of

Dear all,

I am trying to run DESeq2 in R 3.3.1.

When I call library DESeq2 I get this:

"

Loading required package: GenomicRanges
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:parallel’:

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from ‘package:stats’:

    IQR, mad, xtabs

The following objects are masked from ‘package:base’:

    anyDuplicated, append, as.data.frame, cbind, colnames, do.call,
    duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect,
    is.unsorted, lapply, lengths, Map, mapply, match, mget, order,
    paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind,
    Reduce, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Loading required package: S4Vectors
Loading required package: stats4

Attaching package: ‘S4Vectors’

The following objects are masked from ‘package:base’:

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: Rcpp
Loading required package: RcppArmadillo
Warning messages:
1: multiple methods tables found for ‘fileName’ 
2: multiple methods tables found for ‘organism’"

and when I use " DESeqDataSetFromMatrix" function, I get this error message:

"Error in slot(value, what) : 
  no slot of name "exptData" for this object of class "SummarizedExperiment
""

Can u guide me how to solve this problem?

Regards

Nazanin

deseq2 DESeqDataSetFromMatrix software error • 1.1k views
ADD COMMENT
2
Entering edit mode
@mikelove
Last seen 1 day ago
United States

Usually this is a package version problem. Can you run:

library(BiocInstaller)

biocValid()

ADD COMMENT
0
Entering edit mode

Hi Michael,

Yes you are right. The following is what I get when I run  library(BiocInstaller) or biocValid():

downgrade with biocLite(c("AnnotationDbi", "BiocGenerics", "BiocParallel", "Biostrings",
  "bumphunter", "ChAMP", "DESeq2", "fastseg", "GenomicRanges", "genoset",
  "Gviz", "IRanges", "methyAnalysis", "minfi", "pcaMethods", "qvalue",
  "Rsamtools", "XVector"))

Error: 196 package(s) out of date; 18 package(s) too new

Now how can I solve this issue?

ADD REPLY
0
Entering edit mode

Only install with biocLite() and you (should) never have this problem. Try running biocLite() and if this doesn’t work perhaps remove.package() the too new ones.

ADD REPLY
0
Entering edit mode

Thank you so much for your help. It works!

ADD REPLY

Login before adding your answer.

Traffic: 815 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6