I'm currently using the new xcms function which executes the following command
raw_data <- readMSData(files = cdfs, pdata = new("NAnnotatedDataFrame", pd), mode = "onDisk")
The command uses
OnDiskMSnExp object from
MSnbase Bioconductor package. May i know if i made any changes such as retention time alignment using the following command
xdata <- adjustRtime(xdata, param = ObiwarpParam(binSize = 0.6))
Does it overwrites my original files ? or it juz creates a new data in Rstudio environment?