Hello to everyone,
I've followed the "A step-by-step workflow for low-level analysis of single-cell RNA-seq data with Bioconductor, V2" for my own data. The problem is that when I produce the graph for Variance of normalized log-expression values (using trendVar and decomposeVar functions), my plot (an ascending-linear shape in which all of dots are accumulated and correlated with the line of y=x, and it's not at all a bell-shaped graph) is basically different with the plot of workflow (Figure 10). Is it normal?!
I'm wondering if you could give me some hints.