Adjusting DESeq2 p-values through IHW using pathway information as covariate
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@guidobarzaghi-19878
Last seen 2.9 years ago
University of Copenhagen

Greetings everyone,

I have been reading about IHW as a method to adjust p-values for multiple testing by assigning weights to each hypothesis based on a prior (informative) knowledge. My rationale here is that given a list of differentially expressed genes (as returned by DESeq2, but also other methods), I would like to adjust their p-values while accounting for the prior knowledge of which pathway the genes belong to. The idea behind this is that I would expect genes belonging to the same pathways to be more or less co-regulated.

That said, I have immediately faced the harsh truth that most genes are annotated for more then one and sometimes dozens of pathways and so the only way I could come up to pass this information as a vector-like object is to generate a binary string as long as the total number of observed pathways for my list of genes, where each position is either a zero (not belonging) or a one (the gene belongs to that pathway).

I am currently trying this out. Does anyone have a better idea?

Thanks in advance!

IHW deseq2 Pathway • 685 views
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@mikelove
Last seen 14 hours ago
United States

This is novel territory, but you may want to see what the IHW developers have to say. I was thinking earlier that you had a particular pathway of interest.

One way to deal with overlapping categories is if you can order them. This happens in genome annotation, for example, where a region can fall under multiple categories, e.g. intron of one gene vs promoter of another. If you order the categories, you can then come up with a partition.

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