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singhjay
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@singhjay-20324
Last seen 6.0 years ago
Hello, I am using limma package to get the differential gene expression between two groups of the sample run on HGU133 PLUS 2.0 Affymetrix chip. As I am not a bioinformatician so I am struck after normalizing data by Mas5 Algorithm. Can you please suggest me the step by step analysis of data using limma package? Also how to define the two group when I am designing a linear fit model in limma?
Do you've case control study design?