DMDeepm6A: solve_user_SEW0", start, end, width, PACKAGE = "IRanges
0
0
Entering edit mode
jovel_juan ▴ 30
@jovel_juan-7129
Last seen 6 months ago
Canada

Sorry for posting this here. I have tried to post in the github page of DMDeepm6A, but unfortunately en author does not respond, even when I notified through e-mail too.

I am trying to use DMDeepm6A to call methylated bases on RNAseq libraries. The software works beautifully on the toy data provided by the authors of the package (few reads on Chr1).

I have troubleshooted few other problems, but now I am stack near to the end, with the following error message (any help will be highly appreciated):

"---------------------------------" [1] "The bam files used:" [1] "2 IP replicate(s)" [1] "2 Input replicate(s)" [1] "---------------------------------" [1] "Peak calling result: " [1] "2895 peaks detected on merged data." [1] "Please check 'peak.bed/xls' under /home/juan/juan/UofA/projectsanalysis/brunoSaleme/2019/IP190711s1/FunDMDeep-m6A/DMDeepm6Aout/exomepeakuntreated" [1] "1849 consistent peaks detected on every replicates. (Recommended list)" [1] "Please check 'conpeak.bed/xls' under /home/juan/juan/UofA/projectsanalysis/brunoSaleme/2019/IP190711s1/FunDMDeep-m6A/DMDeepm6Aout/exomepeakuntreated" [1] "Detecting sites for IP/Input paire 1 ----------------------------" [1] "Converting BED12 to GRangesList" [1] "It may take a few minutes" [1] "Cutting peak to Single Base..." Time difference of 26.57681 secs [1] "Searching peak for motifs..." Time difference of 32.58991 secs [1] "Making input from peak region..." [1] "Counting reads for single base..." Error in .Call2("solveuser_SEW0", start, end, width, PACKAGE = "IRanges") : solving row 5: range cannot be determined from the supplied arguments (too many NAs)

rtracklayer IRanges DMDeepm6A • 871 views
ADD COMMENT

Login before adding your answer.

Traffic: 561 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6