Hi,

Please suggest any assembled script or steps to RUN DESeq2 to analysis data. I am not sure, what type of file is required for DESeq2. I have output file (count.txt) from FeatureCounts.

Here is some lines of the counts.txt file (output of FeatureCounts). Is that seem correct as input of DESeq?

Geneid  Chr Start   End Strand  Length
ENSSSCG00000048769  1;1;1;1;1   1;792;2371;3118;3118    2465;961;2465;3780;3782 +;+;+;+;+   3130    0
ENSSSCG00000037372  1;1;1;1 9815;14011;18493;22046  10013;15217;18696;22152 -;-;-;- 1717    0

hi sekhwal,

You'll have to start with the tutorials and workflows, we can't go over the material user by user on the support site.

DESeq2 can begin with a count matrix, you'll need to read the data in and create a count matrix and separate sample table.