Dear all, I analyzed RNA-seq data using DESeq2 extracting the results with or without shrinkage
without shrinkage:
res21 <- results(dds2, contrast=c("combined", "XX.DMSO", "XX.dTAG"),
cooksCutoff=FALSE, alpha=0.05)
with shrinkage:
resLFC1 <- lfcShrink(dds2, contrast=c("combined", "XX.DMSO", "XX.dTAG"),
alpha=0.05, type="ashr")
There is a small number of genes were the padj get's NA when including shrinking. Here is a table of the mentioned genes: https://drive.google.com/file/d/1RaHSHuDwtDiUT9-IDmmt3wp-0ImLuY2R/view
Why? Thank's for your help. Best Dagmar
sessionInfo()
R version 3.4.4 (2018-03-15)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Debian GNU/Linux 8 (jessie)
Matrix products: default
BLAS: /usr/lib/libblas/libblas.so.3.0
LAPACK: /usr/lib/lapack/liblapack.so.3.0
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 LC_MONETARY=en_US.UTF-8
[6] LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=C LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] data.table_1.12.0 reshape2_1.4.3 BiocParallel_1.12.0 edgeR_3.20.9 limma_3.34.9
[6] hwriter_1.3.2 ReportingTools_2.17.3 knitr_1.21 genefilter_1.60.0 org.Hs.eg.db_3.5.0
[11] AnnotationDbi_1.40.0 ggplot2_3.1.0 PoiClaClu_1.0.2.1 RColorBrewer_1.1-2 pheatmap_1.0.12
[16] DESeq2_1.18.1 SummarizedExperiment_1.8.1 DelayedArray_0.4.1 matrixStats_0.54.0 Biobase_2.38.0
[21] GenomicRanges_1.30.3 GenomeInfoDb_1.14.0 IRanges_2.12.0 S4Vectors_0.16.0 BiocGenerics_0.24.0
[26] Rsubread_1.28.1
loaded via a namespace (and not attached):
[1] backports_1.1.3 GOstats_2.44.0 Hmisc_4.2-0 AnnotationHub_2.10.1
[5] plyr_1.8.4 lazyeval_0.2.1 GSEABase_1.40.1 splines_3.4.4
[9] digest_0.6.18 invgamma_1.1 BiocInstaller_1.28.0 ensembldb_2.2.2
[13] htmltools_0.3.6 GO.db_3.5.0 SQUAREM_2020.3 magrittr_1.5
[17] checkmate_1.9.1 memoise_1.1.0 BSgenome_1.46.0 cluster_2.0.7-1
[21] Biostrings_2.46.0 annotate_1.56.2 R.utils_2.8.0 ggbio_1.26.1
[25] prettyunits_1.0.2 colorspace_1.4-0 blob_1.1.1 xfun_0.4
[29] dplyr_0.8.0.1 crayon_1.3.4 RCurl_1.95-4.11 graph_1.56.0
[33] survival_2.43-3 VariantAnnotation_1.24.5 glue_1.3.0 gtable_0.2.0
[37] zlibbioc_1.24.0 XVector_0.18.0 Rgraphviz_2.22.0 scales_1.1.1
[41] DBI_1.0.0 GGally_1.4.0 Rcpp_1.0.4.6 xtable_1.8-3
[45] progress_1.2.0 htmlTable_1.13.1 foreign_0.8-71 bit_1.1-14
[49] OrganismDbi_1.20.0 Formula_1.2-3 AnnotationForge_1.20.0 truncnorm_1.0-8
[53] htmlwidgets_1.3 httr_1.4.0 acepack_1.4.1 pkgconfig_2.0.2
[57] reshape_0.8.8 XML_3.98-1.17 R.methodsS3_1.7.1 farver_2.0.3
[61] nnet_7.3-12 locfit_1.5-9.1 tidyselect_0.2.5 labeling_0.3
[65] rlang_0.4.6 later_0.8.0 munsell_0.5.0 tools_3.4.4
[69] RSQLite_2.1.1 evaluate_0.13 stringr_1.4.0 yaml_2.2.0
[73] bit64_0.9-7 purrr_0.3.0 AnnotationFilter_1.2.0 RBGL_1.54.0
[77] mime_0.6 R.oo_1.22.0 biomaRt_2.34.2 compiler_3.4.4
[81] rstudioapi_0.9.0 curl_3.3 interactiveDisplayBase_1.16.0 PFAM.db_3.5.0
[85] tibble_2.0.1 geneplotter_1.56.0 stringi_1.3.1 highr_0.7
[89] GenomicFeatures_1.30.3 lattice_0.20-38 ProtGenerics_1.10.0 Matrix_1.2-15
[93] pillar_1.3.1 lifecycle_0.2.0 bitops_1.0-6 irlba_2.3.3
[97] httpuv_1.4.5.1 rtracklayer_1.38.3 R6_2.4.1 latticeExtra_0.6-28
[101] RMySQL_0.10.16 promises_1.0.1 gridExtra_2.3 dichromat_2.0-0
[105] assertthat_0.2.0 Category_2.44.0 withr_2.1.2 GenomicAlignments_1.14.2
[109] Rsamtools_1.30.0 GenomeInfoDbData_1.0.0 hms_0.4.2 grid_3.4.4
[113] rpart_4.1-13 ashr_2.2-47 biovizBase_1.26.0 mixsqp_0.3-43
[117] shiny_1.2.0 base64enc_0.1-3