EBImage, Image to Signal Conversion
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@kishor-tappita-2563
Last seen 7.7 years ago
Dear All, I am planning to analyze RNAi data with EBImage. I think getFeatures() function converts or extracts the florescent signal intensities. Correct me if I am wrong. The function is returning lot of columns. Can some one help me in understanding the columns as well help me in finding exact signal intensities out of those columns. Thanks in advance. Regards, Kishor
EBImage EBImage • 759 views
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@wolfgang-huber-3550
Last seen 4 months ago
EMBL European Molecular Biology Laborat…
Dear Kishor sorry to be a little bit harsh, but the purpose of this mailing list is not for other people to do your homework for you. The task that you are attempting requires a certain amount of understanding of the underlying algorithm, it is more than just calling a blackbox function. You could have a look at the vignette of the EBImage package and the manual page of the 'getFeatures' function. You will also benefit from reading some of the scientific papers in this field. Kishor Tappita ha scritto: > Dear All, > > I am planning to analyze RNAi data with EBImage. I think getFeatures() > function converts or extracts > the florescent signal intensities. Correct me if I am wrong. > > The function is returning lot of columns. Can some one help me in > understanding the columns as well > help me in finding exact signal intensities out of those columns. > > Thanks in advance. > > Regards, > Kishor > Best wishes Wolfgang ------------------------------------------------------- Wolfgang Huber EMBL http://www.embl.de/research/units/genome_biology/huber
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Dear Wolfgang, Sorry for inconvenience. I should admit that I was a bit lazy and was trying to get my work done soon. I will bear your suggestions in my mind and do the required search. Thanks again for your valuable advice. Thanks, Kishor On Fri, Aug 7, 2009 at 10:37 PM, Wolfgang Huber<whuber at="" embl.de=""> wrote: > > Dear Kishor > > sorry to be a little bit harsh, but the purpose of this mailing list is not > for other people to do your homework for you. The task that you are > attempting requires a certain amount of understanding of the underlying > algorithm, it is more than just calling a blackbox function. You could have > a look at the vignette of the EBImage package and the manual page of the > 'getFeatures' function. You will also benefit from reading some of the > scientific papers in this field. > > Kishor Tappita ha scritto: >> >> Dear All, >> >> I am planning to analyze RNAi data with EBImage. I think getFeatures() >> function converts or extracts >> the florescent signal intensities. Correct me if I am wrong. >> >> The function is returning lot of columns. Can some one help me in >> understanding the columns as well >> help me in finding exact signal intensities out of those columns. >> >> Thanks in advance. >> >> Regards, >> Kishor >> > > > Best wishes > ? ? Wolfgang > > ------------------------------------------------------- > Wolfgang Huber > EMBL > http://www.embl.de/research/units/genome_biology/huber > ------------------------------------------------------- >
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