I tried to load my count matrix with this command:
DESeqObj <- DESeqDataSetFromMatrix(countData = RoundedReadCount,colData = metadata,design = ~ Condition)
After that, I got an error like this:
converting counts to integer mode Error in validObject(.Object) : invalid class “DESeqDataSet” object: NA values are not allowed in the count matrix In addition: Warning message: In `mode<-`(`*tmp*`, value = c(395L, 231L, 243L, 866L, 3L, 45L, : NAs introduced by coercion
I have check in my count matrix there is no NA values. Probably you can give me some suggestion about this problem. Thank you.