ABI HRM data analysis
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Prasanth ▴ 20
Last seen 4 months ago

Hello all, 

I am not an expert in Bioconductor. Recently I started using that after taking a course in HarvardX. I would badly need a package to read High Resolution Melting curve analysis obtained from ABI real-time pcr machine. ABI has a software for that, but it is expensive and unyielding. The file is a normal text file and I can import that into excel sheet and make a curve in excel, if it has less number of samples. I can make a .csv file from excel and import to R. The use mggplot2 or similar graphic package to make the curve. When the sample number increases, it becomes cumbersome. Also, I wanted to automate it and make it more flexible and understandable in R. Can somebody please help me to build this package? Or if anyone has a package, please share with me. I can send the raw files for whoever to try out. 

Many Thanks


ABI sequence DNA melting curve • 794 views
Entering edit mode
Last seen 1 day ago
Seattle, WA, United States

Hi Prasanth,

Sounds like you were able to come up with something that more or less works. If not, please be more specific about what problems you're facing. Also the bioc-devel mailing list (http://bioconductor.org/help/support/#bioc-devel) is a better place to ask questions about package development. Note that developing a package is a full project: it can be challenging and time consuming. Asking that somebody helps you build the package is almost like asking that somebody helps you write a paper. It's fine, but that means establishing a close collaboration with someone and/or sometimes hiring someone.




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