After trying some R scripts to generate DEG without success, I come here to make a simple question, where can I find an R script that calculates the differentiation expression of genes?
Recently I found a script in a book called Statistics and Data Analysis for Microarrays Using R and Bioconductor, and I tried the scripts found in that book, after some lines of code, the script stoped.
I tried also the generated script in GEO2R, I selected the serie and created 2 groups, normal and cancer, and started to make the comparison. I copied the code in the website and tried to reproduce in my pc, but without success, says an error about the object doesn´t have an numeric value. I emailed GEO2R about that, and I received a response saying that the developers were informed about it, hoping to hear from them soon. If the script of GEO2R works in local machines, I could use for all the series that I need, assuming that this script is reliable.
I´m trying to check DEG in normal an cancer cells, I appreciate the attention.
P.S - I modified all the R scripts that I came across to my needs, and made some modifications to see if I could solve it, but with no sucess until now.