I have received data from a novocyte cytometer which uses 24-bit data. Turns out most of the data in the negative population is clustered around zero and a lot of it is negative. How can I display this data using xyplot or plotgate?
xyplot or plotGate removes the marginal events by default, you can disable this behavior by passing 'margin = FALSE' option to them.
You can also specify the axis ranges , e.g.
plotGate(gs[], "CD4", margin = F, ylim = c(-1e3, 3e3))
Thanks for the tip. margin=F did the trick. The data presentation looks good using xlim="data" or a custom range. My problem now is that the data range displayed on both the x and y axes are not negative. All values are positive. Is there a way to adjust the displayed range?
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