Question: Displaying Negative data from flow cytometers
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gravatar for arnaud.colantonio
4.1 years ago by
United States
arnaud.colantonio0 wrote:

I have received data from a novocyte cytometer which uses 24-bit data.  Turns out most of the data in the negative population is clustered around zero and a lot of it is negative.  How can I display this data using xyplot or plotgate?

ADD COMMENTlink modified 4.1 years ago by Jiang, Mike1.2k • written 4.1 years ago by arnaud.colantonio0
Answer: Displaying Negative data from flow cytometers
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gravatar for Jiang, Mike
4.1 years ago by
Jiang, Mike1.2k
(Private Address)
Jiang, Mike1.2k wrote:

xyplot or plotGate removes the marginal events by default, you can disable this behavior by passing 'margin = FALSE' option to them.

ADD COMMENTlink modified 4.1 years ago • written 4.1 years ago by Jiang, Mike1.2k

You can also specify the axis ranges , e.g. 

plotGate(gs[[1]], "CD4", margin = F, ylim = c(-1e3, 3e3))
ADD REPLYlink written 4.1 years ago by Jiang, Mike1.2k

Thanks for the tip.  margin=F did the trick.  The data presentation looks good using xlim="data" or a custom range.  My problem now is that the data range displayed on both the x and y axes are not negative.  All values are positive.  Is there a way to adjust the displayed range?

ADD REPLYlink written 4.1 years ago by arnaud.colantonio0
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