Displaying Negative data from flow cytometers
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@arnaudcolantonio-8998
Last seen 6.3 years ago
United States

I have received data from a novocyte cytometer which uses 24-bit data.  Turns out most of the data in the negative population is clustered around zero and a lot of it is negative.  How can I display this data using xyplot or plotgate?

flowcore flowworkspace flowviz • 846 views
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Jiang, Mike ★ 1.3k
@jiang-mike-4886
Last seen 3 months ago

xyplot or plotGate removes the marginal events by default, you can disable this behavior by passing 'margin = FALSE' option to them.

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You can also specify the axis ranges , e.g.

plotGate(gs[[1]], "CD4", margin = F, ylim = c(-1e3, 3e3))
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Thanks for the tip.  margin=F did the trick.  The data presentation looks good using xlim="data" or a custom range.  My problem now is that the data range displayed on both the x and y axes are not negative.  All values are positive.  Is there a way to adjust the displayed range?