I am trying to use flowCore's read.FCS() to import data into an R session.  When I do this, I get the following error:

library(flowCore)

fs <- list.files(path="~/data_temp", pattern="fcs", full.names=TRUE)

!> read.FCS(fs[[1]])

Error in .readFCSdataRaw(con, dattype, count = as.integer(offsets["dataend"] - : object 'splitInt' not found

However, when I try do the following, I do not get this error:

> library(flowClean) > data(synPerturbed) > write.FCS("~/Desktop/temp.fcs")

[1] "~/Desktop/temp.fcs" Warning message: In write.FCS(synPerturbed, "~/Desktop/temp.fcs") : 'write.FCS' is not fully tested and should be considered as experimental. !> read.FCS("~/Desktop/temp.fcs") flowFrame object '9301d9e4-a160-477f-a5fb-ee7d785d5655' with 76466 cells and 17 observables: name desc range minRange maxRange $P1 FSC-A <NA> 262144 0.00000 262144 $P2 FSC-H <NA> 262144 0.00000 262144 $P3 SSC-A <NA> 261589 0.00000 261589 $P4 Time <NA> 2048 0.00000 2048 $P5 <B515-A> CD27 FITC 260954 -26.88460 260954 $P6 <V705-A> CD57 QD705 261947 -111.00000 261947 $P7 <G560-A> CD95 PE 261196 -111.00000 261196 $P8 <G660-A> CD28 Cy5 PE 261507 -111.00000 261507 $P9 <G710-A> CD244 Cy55 PE 261763 -111.00000 261763 $P10 <G780-A> CD31 Cy7 PE 261402 -111.00000 261402 $P11 <R710-A> CCR7 Ax680 261602 -111.00000 261602 $P12 <R780-A> CD3 H7 APC 261301 -111.00000 261301 $P13 <V450-A> CD127 BV421 260964 -35.98385 260964 $P14 <V545-A> Aq Blu 260949 -22.20724 260949 $P15 <V585-A> CD8 QD585 261965 -111.00000 261965 $P16 <R660-A> CD45RA APC 261023 -96.50925 261023 $P17 <V605-A> CD4 BV605 261131 -111.00000 261131 212 keywords are stored in the 'description' slot

That is, this problem happens sometimes.  Any troubleshooting advice would be appreciated.

!> sessionInfo() R version 3.2.2 (2015-08-14) Platform: x86_64-pc-linux-gnu (64-bit) Running under: Ubuntu 14.04.3 LTS

locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C

attached base packages: [1] stats graphics grDevices utils datasets methods base

other attached packages: [1] flowClean_1.6.0 flowCore_1.36.0

loaded via a namespace (and not attached): [1] Rcpp_0.12.1 lattice_0.20-33 mvtnorm_1.0-3 [4] changepoint_2.1.1 corpcor_1.6.8 zoo_1.7-12 [7] rrcov_1.3-8 grid_3.2.2 stats4_3.2.2 [10] pcaPP_1.9-60 graph_1.48.0 robustbase_0.92-5 [13] tools_3.2.2 Biobase_2.30.0 bit_1.1-12 [16] DEoptimR_1.0-4 sfsmisc_1.0-28 parallel_3.2.2 [19] compiler_3.2.2 BiocGenerics_0.16.0 cluster_2.0.3

Thanks Mike, this works now!