Plotting flow cytometry data
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gori73 • 0
@gori73-9176
Last seen 6.5 years ago
United States

Hello,

 

 

 I am  new  to R and would appreciate your help.

 

I am using flowcore package to load my FCS files to R but then extract the relevant data to a regular dataframe

as I am not familiar with the manipulation of S4 type data.

 

I am currently using smoothscatter function to display my data but would like to produce non-smoothed  image more similar to FlowJo.

I like the images produced by the examples in  FlowViz vignette but don’t know how to use on my simple dataframe.

When I use the code below I get just a one color plot instead of different colors representing cell densities.

 

library(flowViz)
require(IDPmisc)

colramp <- colorRampPalette(IDPcolorRamp(21))

xyplot(DNAA~YFP, data, nbin = 100, smooth=FALSE, colramp=colramp)

 

In this case DNAA and YFP are two columns in the dataframe “data”.

 

How should I modify my code to get a plot similar to the one in FlowViz vignette? (use on simple dataframes)

 

Thank you very much,

 

Igor

flowviz flowcore • 926 views
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Entering edit mode
Jiang, Mike ★ 1.3k
@jiang-mike-4886
Last seen 7 months ago
(Private Address)

You must use flowSet/flowFrame in order to take advantage of all the features provided by flowViz package.  If you convert it to a data.frame,  then it is simply a generic visualization question of 'lattice' or 'ggplot2' which is beyond the scope of flowViz or flow cytometry. But I can help you with this particular one, 

 

cols <- densCols(x = data[["DNAA"]], y = data[["YFP"]], colramp=colramp)

xyplot(`SSC-H`~`FSC-H`, data,  col = cols)

 

 

Again, this is not an idea way of using flowCore. What kind of data manipulation are you talking about?I would say at least you can assign the data back to flowSet/flowFrame object before plotting them. See

?`exprs<-`
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