I am new to R and would appreciate your help.
I am using flowcore package to load my FCS files to R but then extract the relevant data to a regular dataframe
as I am not familiar with the manipulation of S4 type data.
I am currently using smoothscatter function to display my data but would like to produce non-smoothed image more similar to FlowJo.
I like the images produced by the examples in FlowViz vignette but don’t know how to use on my simple dataframe.
When I use the code below I get just a one color plot instead of different colors representing cell densities.
colramp <- colorRampPalette(IDPcolorRamp(21))
xyplot(DNAA~YFP, data, nbin = 100, smooth=FALSE, colramp=colramp)
In this case DNAA and YFP are two columns in the dataframe “data”.
How should I modify my code to get a plot similar to the one in FlowViz vignette? (use on simple dataframes)
Thank you very much,