I have worked through the WGCNA tutorial I using my own data and I have used method 2b to construct my network and form modules and i have progressed to stage 3 where I've applied my traits and I have found some interesting findings.
I now want to look at hub genes from within those modules significantly associated with my trait of interest but when I move to the Tutorial III section I find that the input files bear no relationship to the files saved from the Tutorial I. Specifically, I am struggling to find a match from my data that corresponds to ModuleEigengeneNetwork1, GeneSummaryTutorial.csv, and TraitsTutorial.csv.
My aim is to get to Tutorial III part 7 and any guidance as to how I can get the data from Tutorial I to match the required input for Tutorial III would be greatly appreciated.
Many thanks - Hywel