Error in Matrix
1
0
Entering edit mode
rasii • 0
@rasii-10044
Last seen 8.2 years ago

Hi all,

I am analysing the new Illumina EPIC methylation array and I keep getting the following error. Can anyone help me figure what/where I've gone wrong. Below is the code I have used:

>require(minfi)

>targets <- read.metharray.sheet(baseDir)

> RGset <- read.metharray.exp(targets = targets)

> RGset@annotation = c(array = "IlluminaHumanMethylationEPIC", annotation = "ilm10b2.hg19")

>RGset

RGChannelSet (storageMode: lockedEnvironment)
assayData: 1052641 features, 8 samples 
  element names: Green, Red 
An object of class 'AnnotatedDataFrame'
  sampleNames: 1 2 ... 134 (134 total)
  varLabels: Sample_Name Sample_Group ... Basename (9
    total)
  varMetadata: labelDescription
Annotation
  array: IlluminaHumanMethylationEPIC
  annotation: ilm10b2.hg19

>pd <- pData(RGset)

> densityPlot(RGset, sampGroups = pd$Sample_Group,
+ main = "Beta", xlab = "Beta")
Error in matrix(NA_real_, ncol = ncol(rgSet), nrow = length(locusNames),  : 
  length of 'dimnames' [2] not equal to array extent

> densityBeanPlot(RGset, sampGroups = pd$Sample_Group)
Error in matrix(NA_real_, ncol = ncol(rgSet), nrow = length(locusNames),  : 
  length of 'dimnames' [2] not equal to array extent

> raw <- preprocessRaw(RGset)
Error in matrix(NA_real_, ncol = ncol(rgSet), nrow = length(locusNames),  : 
  length of 'dimnames' [2] not equal to array extent

I cant seem to be able to plot anything and it doesnt even let me process the data. What am I doing wrong? help please..

Rasi

P.S Im using the latest version of R (beta) and minfi (1.17.10)

minfi methylation EPIC microarray illumina • 1.8k views
ADD COMMENT
0
Entering edit mode
@kasper-daniel-hansen-2979
Last seen 17 months ago
United States
Well, it looks like a bug. Could you start an issue on the Github page? On Sat, Apr 23, 2016 at 3:07 AM, rasii [bioc] <noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User rasii <https: support.bioconductor.org="" u="" 10044=""/> wrote Question: > Error in Matrix <https: support.bioconductor.org="" p="" 81199=""/>: > > Hi all, > > I am analysing the new Illumina EPIC methylation array and I keep getting > the following error. Can anyone help me figure what/where I've gone wrong. > Below is the code I have used: > > *>require*(minfi) > > >targets <- read.metharray.sheet(baseDir) > > > RGset <- read.metharray.exp(targets = targets) > > > RGset@annotation = c(array = "IlluminaHumanMethylationEPIC", annotation > = "ilm10b2.hg19") > > >RGset > > RGChannelSet (storageMode: lockedEnvironment) > assayData: 1052641 features, 8 samples > element names: Green, Red > An object of class 'AnnotatedDataFrame' > sampleNames: 1 2 ... 134 (134 total) > varLabels: Sample_Name Sample_Group ... Basename (9 > total) > varMetadata: labelDescription > Annotation > array: IlluminaHumanMethylationEPIC > annotation: ilm10b2.hg19 > > >pd <- pData(RGset) > > > densityPlot(RGset, sampGroups = pd$Sample_Group, > + main = "Beta", xlab = "Beta") > > *Error in matrix(NA_real_, ncol = ncol(rgSet), nrow = length(locusNames), > : length of 'dimnames' [2] not equal to array extent* > > densityBeanPlot(RGset, sampGroups = pd$Sample_Group) > > *Error in matrix(NA_real_, ncol = ncol(rgSet), nrow = length(locusNames), > : length of 'dimnames' [2] not equal to array extent* > > raw <- preprocessRaw(RGset) > > *Error in matrix(NA_real_, ncol = ncol(rgSet), nrow = length(locusNames), > : length of 'dimnames' [2] not equal to array extent* > > I cant seem to be able to plot anything and it doesnt even let me process > the data. What am I doing wrong? help please.. > > Rasi > > P.S Im using the latest version of R (beta) and minfi (1.17.10) > > ------------------------------ > > Post tags: minfi, methylation, EPIC microarray, illumina > > You may reply via email or visit Error in Matrix >
ADD COMMENT

Login before adding your answer.

Traffic: 589 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6