I am trying to find all gRNAs in my target sequence of interest.
1.Am I right in thinking that provided that there is no annotation error I should get at least one hit with 0 mismatch for each generated gRNA, which correspond to my target sequence ?
2.Also, since my target sequence is very long I find it hard to discriminate between gRNAs - which criteria should I look for ? gRNA efficiency ? But as > 1 off targets are listed for each gRNAs, how should this value be used ? Should I be using the Top?OfftargetTotalScore in the Summary file instead ?