WGCNA - Network construction and module detection
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Recep ▴ 20
@7c8fd686
Last seen 2.1 years ago
Germany

Hello im using WGCNA to cluster genes. I got this tutorial code from here.

I got the concept and how to choice the power but I am wondering, how can i select the parameters of power (like here from 10 to 30 and c is between 1 and 10) and h, which is here 0.90.

    powers = c(c(1:10), seq(from =10, to=30, by=1)) #choosing a set of soft-thresholding powers
    sft = pickSoftThreshold(datExpr, powerVector=powers, verbose =5, networkType="signed") #call network enter code heretopology analysis function

    sizeGrWindow(9,5)
    par(mfrow= c(1,2))
    cex1=0.9
    plot(sft$fitIndices[,1], -sign(sft$fitIndices[,3])*sft$fitIndices[,2], xlab= "Soft Threshold (power)", ylab="Scale Free Topology Model Fit, signed R^2", type= "n", main= paste("Scale independence"))
    text(sft$fitIndices[,1], -sign(sft$fitIndices[,3])*sft$fitIndices[,2], labels=powers, cex=cex1, col="red")
    abline(h=0.90, col="red")
    plot(sft$fitIndices[,1], sft$fitIndices[,5], xlab= "Soft Threshold (power)", ylab="Mean Connectivity", type="n", main = paste("Mean connectivity"))
    text(sft$fitIndices[,1], sft$fitIndices[,5], labels=powers, cex=cex1, col="red")

    #from this plot, we would choose a power of 18 becuase it's the lowest power for which the scale free topology index reaches 0.90
gene Clustering wgcna flashclust • 1.1k views
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