Merging 2 staining panels
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@473e249a
Last seen 14 months ago
United Kingdom

Hello, I'm quite new to flow data analysis in R and I'm trying to merge 2 staining panels. I have 58 patients samples, but 34 were processed with panel1 and after that they added 2 extra markers in the panel (into the free channels) and then run another 24 samples. R won't allow me to create a flowset of all as the colnames don't match. I've created separate ncdfFlowLists for those panels, but I don't know how to select specific markers and merge. Is there a way to merge two FlowLists based on specific markers? Or do I need to make some steps before this? Thank you!


ncdflist <- ncdfFlowList(list(FlowList1, FlowList2))

FlowSet1_2 <- ncdfFlow::rbind2(ncdflist)

Error: The colnames of the ncdfFlowSets don't match.

FlowCytometry flowCore • 347 views
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