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I am trying to analyze a single cell sequence using GSE148434 data.
I want to analyze using the 'Seurat' package, but I don't know how to do it.
I succeeded in loading the 'h5' format file. Here is my code.
untar("GSE148434_h5_RAW.tar")
h5.fileList <- list.files(pattern = "info.h5")
load_h5_file <- function(h5.fileList) {
h5_data <- h5read(h5.fileList, "/")
return(h5_data)
}
all_h5_data <- lapply(h5.fileList, load_h5_file)
And this is one of the data examples included in 'all_h5_data'. 'all_h5_data' is a 'Large list' format in R.
I don't know how to perform single cell rna sequencing analysis using the 'Seurat' package.
Please help me.