'Seurat' package
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Sooni ▴ 10
@fcaec06f
Last seen 3 months ago
South Korea

I am trying to analyze a single cell sequence using GSE148434 data.

I want to analyze using the 'Seurat' package, but I don't know how to do it.

I succeeded in loading the 'h5' format file. Here is my code.

untar("GSE148434_h5_RAW.tar")
h5.fileList <- list.files(pattern = "info.h5")
load_h5_file <- function(h5.fileList) {
  h5_data <- h5read(h5.fileList, "/")
  return(h5_data)
}
all_h5_data <- lapply(h5.fileList, load_h5_file)

And this is one of the data examples included in 'all_h5_data'. 'all_h5_data' is a 'Large list' format in R.

enter image description here

I don't know how to perform single cell rna sequencing analysis using the 'Seurat' package.

Please help me.

singlecell seurat • 998 views
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3
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ATpoint ★ 4.5k
@atpoint-13662
Last seen 3 days ago
Germany

Seurat is not a Bioconductor package. Please read their documentation and guided tutorials for starters.

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