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Yuko
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Answer: DESeq2 + analysis question for design with uneven time point design
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Michael Love
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I'd recommend reaching out to a local statistician or someone familiar with linear models in R. Unfortunately, I have to reserve my time on…
Answer: Genes with highest differential expression have very low read counts for most ge
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Michael Love
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Have you use `lfcShrink`? Or `results` with `lfcThreshold`? Either of these are recommended for prioritizing DE genes.
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Gordon Smyth
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> equivqalent in limma would be like a gene by gene specificic design matrix where no BL time point would exist but a continuous variable t…
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ATpoint
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Yes, with normalized counts on log2 scale.
Comment: Adjusting for baseline when having longitudinal data, mixed model and no treat
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Anna
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A no I never referred to transcriptomic data when I was talking about "standard procedure"
I was referring to the way we analyze longitudin…
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