Question

I changed the reference in DGE contrasts by DESeq2, but the results changed just a bit and not in the sign of Log2FC (positive to negative and inverse) as I expected. Why?

0

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mohammad.hossein.banabazi • 0

@3f59e3b4

Last seen 9 months ago

Sweden

Enter the body of text here

Code should be placed in three backticks as shown below


# include your problematic code here with any corresponding output 
# please also include the results of running the following in an R session 

sessionInfo( )

RNASeqData DESeq2 • 513 views

ADD COMMENT • link updated 9 months ago by Michael Love 41k • written 9 months ago by mohammad.hossein.banabazi • 0

1

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Hi, welcome. Please understand that a question being in the title, without any text or code, plots etc is not sufficient to diagnose the issue. Please edit accordingly.

ADD REPLY • link 9 months ago ATpoint ★ 4.0k

score 0 · Answer 1 · 2023-07-24

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Michael Love 41k

@mikelove

Last seen 5 hours ago

United States

You didn't provide code, but I'm guessing the results didn't change sign bc you used contrast.

ADD COMMENT • link 9 months ago Michael Love 41k