I'm new in RNA-seq analysis and would like to seek some guidance on the data analysis.
My experiment design has two variables "Concentration" and "Addition". I have 8 samples in total.
There are 4 concentrations of chemical X was added in experiments which is 0, 25, 50, 75 and 100 under "Concentration".
2 values Y and N stands for with and without nutrients under "Additional nutrients".
I have to understand, when adding additional nutrients to 4 different concentrations of chemicals, any overlaped/common genes are up/down-regulated.
design = ~ Additional nutrients + Concentration design = ~ Additional nutrients + Concentration + Additional nutrients:Concentration design = ~ Additional nutrients + Additional nutrients:Concentration
Can I know which one is correct?
Code should be placed in three backticks as shown below