Hi everyone, I am a novice in the enrichment analyses and I have a problem with the gseGO function of the clusterprofiler package in R. Basically I'm analyzing mouse expression data and when I go to run the analysis with my code there seem to be only enriched pathways (with positive NES). I don't understand why if I use the human annotation org.Hs.eg.db my results and graphs come out as I expect them to. The first plot comes from the following code with mouse annotation (org.Mm.eg.db) and the second plot comes from the code with the human annotation.

GO_gsea <- function(df) { 
                     library(clusterProfiler) 
                     library(org.Mm.eg.db) 
                     geneList = df[,2] 
                     names(geneList) = as.character(df[,7]) 
                     geneList = sort(geneList, decreasing = TRUE) 
                     data_GO_GSEA <- gseGO(geneList = geneList, OrgDb = org.Mm.eg.db, ont = "BP", 
                                                     minGSSize = 3, maxGSSize = 800, pvalueCutoff = 0.05, verbose = TRUE,  
                                                     keyType = "SYMBOL", pAdjustMethod = "BH", eps = 0) }
CeO2vsDiff1_gsea <- GO_gsea(CeO2vsDiff1) 
dotplot(CeO2vsDiff1_gsea, showCategory=10, split=".sign") + facet_grid(.~.sign)

sessionInfo( )
R version 4.3.1 (2023-06-16)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Sonoma 14.4

Matrix products: default
BLAS:   /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/lib/libRlapack.dylib;  LAPACK version 3.11.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: Europe/Rome
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] BiocManager_1.30.22   org.Hs.eg.db_3.17.0   lubridate_1.9.3       forcats_1.0.0        
 [5] stringr_1.5.1         dplyr_1.1.4           purrr_1.0.2           readr_2.1.5          
 [9] tidyr_1.3.1           tibble_3.2.1          tidyverse_2.0.0       org.Mm.eg.db_3.17.0  
[13] AnnotationDbi_1.62.2  IRanges_2.34.1        S4Vectors_0.38.2      Biobase_2.60.0       
[17] BiocGenerics_0.46.0   ggplot2_3.5.0         enrichplot_1.20.3     clusterProfiler_4.8.3

loaded via a namespace (and not attached):
 [1] DBI_1.2.2               bitops_1.0-7            gson_0.1.0             
 [4] shadowtext_0.1.3        gridExtra_2.3           rlang_1.1.3            
 [7] magrittr_2.0.3          DOSE_3.26.2             compiler_4.3.1         
[10] RSQLite_2.3.5           png_0.1-8               vctrs_0.6.5            
[13] reshape2_1.4.4          pkgconfig_2.0.3         crayon_1.5.2           
[16] fastmap_1.1.1           XVector_0.40.0          labeling_0.4.3         
[19] ggraph_2.2.1            utf8_1.2.4              HDO.db_0.99.1          
[22] tzdb_0.4.0              bit_4.0.5               zlibbioc_1.46.0        
[25] cachem_1.0.8            aplot_0.2.2             jsonlite_1.8.8         
[28] GenomeInfoDb_1.36.4     blob_1.2.4              BiocParallel_1.34.2    
[31] tweenr_2.0.3            parallel_4.3.1          R6_2.5.1               
[34] stringi_1.8.3           RColorBrewer_1.1-3      GOSemSim_2.26.1        
[37] Rcpp_1.0.12             downloader_0.4          timechange_0.3.0       
[40] Matrix_1.6-5            splines_4.3.1           igraph_2.0.3           
[43] tidyselect_1.2.1        qvalue_2.32.0           rstudioapi_0.15.0      
[46] viridis_0.6.5           codetools_0.2-19        lattice_0.22-6         
[49] plyr_1.8.9              treeio_1.24.3           withr_3.0.0            
[52] KEGGREST_1.40.1         gridGraphics_0.5-1      scatterpie_0.2.1       
[55] polyclip_1.10-6         Biostrings_2.68.1       pillar_1.9.0           
[58] ggtree_3.8.2            ggfun_0.1.4             generics_0.1.3         
[61] RCurl_1.98-1.14         hms_1.1.3               munsell_0.5.0          
[64] scales_1.3.0            tidytree_0.4.6          glue_1.7.0             
[67] lazyeval_0.2.2          tools_4.3.1             data.table_1.15.2      
[70] fgsea_1.26.0            fs_1.6.3                graphlayouts_1.1.1     
[73] fastmatch_1.1-4         tidygraph_1.3.1         cowplot_1.1.3          
[76] grid_4.3.1              ape_5.7-1               colorspace_2.1-0       
[79] nlme_3.1-164            GenomeInfoDbData_1.2.10 patchwork_1.2.0        
[82] ggforce_0.4.2           cli_3.6.2               fansi_1.0.6            
[85] viridisLite_0.4.2       gtable_0.3.4            yulab.utils_0.1.4      
[88] digest_0.6.35           ggrepel_0.9.5           ggplotify_0.1.2        
[91] farver_2.1.1            memoise_2.0.1           lifecycle_1.0.4        
[94] httr_1.4.7              GO.db_3.17.0            bit64_4.0.5            
[97] MASS_7.3-60.0.1        
Error in exists(cacheKey, where = .rs.WorkingDataEnv, inherits = FALSE) : 
  invalid first argument