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Question: Extract the gate in the data file (fcs file) from MoFlo Astrios
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16 months ago by
celine.jouanin0 wrote:

Hello,

Is it possible to know the gate (polygon) created in a strategy from MoFlo Astrios cytometer analysis, to extract the data we want ?

I suppose the coordinates of the gate from MoFlo Astrios analysis are with the data in the fcs file, but I don't succeed to have it.

Thanks

Céline

written 16 months ago by celine.jouanin0

So far we've developed and supported parsing gating strategy/hierarchy) from the xml workspaces generated by flowJo, cytobank and diva (winlist is currently under development). See https://github.com/RGLab/CytoML and https://github.com/RGLab/flowWorkspace

I am not sure how your software stores the gates.  Can you send along the example fcs for me to take a look? I am not quite sure if FCS is designed for that.