I have a question about the the off target region.
suppose the locus is 1000 for a target frame of 20bp in the sequence.
Now what will be the region of consideration for the off target analysis.
What i understood from papers and links, that it should be plus/minus 500bp from target region at max.
Shall I consider the the locus from 500bp to 1500bp to find out all potential of target for the target frame at location 1000.
If I am wrong please guide me.