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Comment: Merge Replicates vs Mixed Model
by
gabriel.hoffman
▴ 170
For your proteomics data, your normalization is correct. I had assumed it was RNA-seq because that is what I think about most. When using…
Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
mohammedtoufiq91
▴ 10
@gordonsmyth I will re-run Salmon with the --numGibbsSamples=50 option. Thank you.
Comment: Differential Expression Genes as Independent Variables
by
Gordon Smyth
52k
Such an analysis cannot be done in limma. Sorry, I cannot tell you how to do it or even whether it is possible.
Comment: Differential Expression Genes as Independent Variables
by
abadgerw
• 0
The data that is repeated is a quantitative metric of post-mortem pathology across multiple sections. Not all patients have the same number…
Answer: Crossover design with limma
by
Gordon Smyth
52k
Your experiment is somewhat more elaborate than a classical crossover design because of the period-specific baseline controls. The analyses…
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Comment: What type of counts data to import for performing Isoform analysis in edgeR
Answer: What type of counts data to import for performing Isoform analysis in edgeR
Transcript-level differential expression using DESeq2
Comment: What type of counts data to import for performing Isoform analysis in edgeR
Answer: What type of counts data to import for performing Isoform analysis in edgeR
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