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Answer: Automatically generate contrasts from a model matrix / formula by Gordon Smyth 51k
I am very concerned that you are attempting to extract pairwise comparisons from an interaction formula like `0 + Group*Sex + Age`, because…
Comment: Mostly increased counts in differentially accessible peaks using DESeq2 by Ricardo Iván • 0
Hi Michael, Thanks for your reply. After reviewing the PCAs, maybe the issue that despite the replicates looking similar, there is still a…
Comment: Using Normalized Counts in DESeq2 by Michael Love 42k
Also, just to add, there are many dozens of posts on this topic of DESeq2 applied to TCGA counts, normalized or other. Recommend google sea…
Answer: Using Normalized Counts in DESeq2 by ATpoint ★ 4.5k
TCGA raw counts can be obtained for example using the TCGAbiolinks or recount packages.
Comment: How to reverse complement some elements of a DNAStringSet by Christine Jones • 0
Fantastic, thank you
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Answer: When running contrasts, does it use normalized read counts?
Comment: featureCounts fails to load annotation file
Answer: How to reverse complement some elements of a DNAStringSet
Answer: KEGGgraph result difference
Answer: What should be the normalization protocol for RNA seq data for WGCNA?
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