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deseq2
•
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1
vote
2
replies
153
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
DESeq2
eigencorplot
PCAtools
3 days ago • updated 8 hours ago
BioinfGuru
▴ 30
4
votes
9
replies
212
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 15 hours ago by
ATpoint
★ 4.1k • written 1 day ago by
pangtaihin
• 0
0
votes
3
replies
87
views
Assymetric MA plot with no overall trend
DESeq2
DifferentialExpression
updated 1 day ago by
Michael Love
42k • written 2 days ago by
Kasper
• 0
0
votes
8
replies
460
views
Can I trust the differential expression analysis when the two groups overlap on the PCA?
DESeq2
pcaExplorer
updated 2 days ago by
BioinfGuru
▴ 30 • written 3 days ago by
Eric Katagirya
• 0
0
votes
1
reply
73
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 2 days ago by
Michael Love
42k • written 3 days ago by
piemmea
• 0
2
votes
7
replies
352
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
8 days ago • updated 5 days ago
sajadahmad41454
• 0
0
votes
2
replies
1.5k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 6 days ago by
maria.soler
• 0 • written 15 months ago by
kalavattam
▴ 10
0
votes
1
reply
152
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 6 days ago by
ATpoint
★ 4.1k • written 6 days ago by
sajadahmad41454
• 0
0
votes
1
reply
95
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 7 days ago by
ATpoint
★ 4.1k • written 7 days ago by
sajadahmad41454
• 0
1
vote
3
replies
300
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 8 days ago by
ATpoint
★ 4.1k • written 15 days ago by
angelathuynh5
• 0
1
vote
1
reply
184
views
Handling multiple differential expression comparisons
DESeq2
updated 11 days ago by
Michael Love
42k • written 13 days ago by
Zainab
▴ 10
2
votes
3
replies
251
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 11 days ago by
ATpoint
★ 4.1k • written 13 days ago by
Reza
• 0
0
votes
6
replies
281
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 12 days ago by
swbarnes2
★ 1.4k • written 14 days ago by
arfranco
▴ 130
0
votes
2
replies
164
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
16 days ago • updated 13 days ago
Aurora
• 0
0
votes
2
replies
204
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
15 days ago • updated 14 days ago
kcarey
• 0
0
votes
2
replies
157
views
DESeq2 v1.35.0
DESeq2
updated 14 days ago by
Michael Love
42k • written 14 days ago by
Espresso
• 0
0
votes
3
replies
208
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 14 days ago by
ATpoint
★ 4.1k • written 15 days ago by
james.zhang20
• 0
0
votes
3
replies
298
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 15 days ago by
Michael Love
42k • written 16 days ago by
HAK
• 0
1
vote
4
replies
518
views
DEseq2 coefficient
DESeq2
coefficient
11 weeks ago • updated 15 days ago
JKim
• 0
6
votes
19
replies
2.5k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 16 days ago by
Sey R.
• 0 • written 4.6 years ago by
hsbio
▴ 10
1
vote
1
reply
150
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 16 days ago by
Michael Love
42k • written 16 days ago by
marcusn
• 0
0
votes
1
reply
135
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 16 days ago by
Michael Love
42k • written 17 days ago by
Agung
• 0
0
votes
1
reply
134
views
putting my own expression data in deseq2
DESeq2
updated 16 days ago by
ATpoint
★ 4.1k • written 16 days ago by
Dev
• 0
3
votes
3
replies
264
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 17 days ago by
ATpoint
★ 4.1k • written 17 days ago by
bioinf
▴ 10
0
votes
2
replies
225
views
PCA plot suggestions
DESeq2
PCAtools
updated 20 days ago by
Michael Love
42k • written 22 days ago by
Aaliya
• 0
1
vote
2
replies
737
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
29 days ago • updated 20 days ago
Patadù
▴ 10
1
vote
3
replies
250
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 20 days ago by
Michael Love
42k • written 21 days ago by
JalapenoCornbread
• 0
1
vote
4
replies
353
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
22 days ago • updated 20 days ago
winwater0928
• 0
2
votes
9
replies
423
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 21 days ago by
James W. MacDonald
65k • written 21 days ago by
Alexandre
• 0
0
votes
8
replies
379
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 21 days ago by
swbarnes2
★ 1.4k • written 23 days ago by
Aaliya
• 0
0
votes
1
reply
165
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 22 days ago by
Michael Love
42k • written 22 days ago by
kcarey
• 0
1
vote
3
replies
234
views
Different Shrunk Log2FC according with reference
DESeq2
updated 22 days ago by
Michael Love
42k • written 22 days ago by
andrebolerbarros
▴ 20
3
votes
1
reply
328
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 23 days ago by
Michael Love
42k • written 23 days ago by
i.sudbery
▴ 40
3
votes
3
replies
249
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 23 days ago by
Michael Love
42k • written 24 days ago by
WardDeb
• 0
0
votes
4
replies
261
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
29 days ago • updated 26 days ago
lonn
• 0
1
vote
2
replies
259
views
How to retrieve detailed regression results from deseq2
DESeq2
5 weeks ago • updated 27 days ago
Weiqian
▴ 10
0
votes
7
replies
444
views
DESeq filtering specific to contrasts
DESeq2
updated 27 days ago by
Michael Love
42k • written 3 months ago by
Emma
• 0
2
votes
5
replies
788
views
DESeq2 setting significant p-value
DESeq2
updated 27 days ago by
Michael Love
42k • written 5.0 years ago by
Merlin
▴ 10
0
votes
4
replies
291
views
DEG with low counts
DESeq2
updated 27 days ago by
Michael Love
42k • written 4 weeks ago by
Rajat
• 0
0
votes
1
reply
153
views
rlog transformation
DESeq2
updated 28 days ago by
Michael Love
42k • written 28 days ago by
ishij9717
• 0
0
votes
1
reply
124
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 28 days ago by
Michael Love
42k • written 28 days ago by
09191919
• 0
0
votes
1
reply
149
views
covariate with negative and positive values
DESeq2
updated 28 days ago by
Michael Love
42k • written 4 weeks ago by
Brynn
• 0
9
votes
12
replies
7.9k
views
Multi factor design edgeR/Deseq2
deseq2
edgeR
rna-seq
updated 28 days ago by
Elizabeth
• 0 • written 8.9 years ago by
gthm
▴ 30
0
votes
1
reply
163
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 28 days ago by
ATpoint
★ 4.1k • written 29 days ago by
quanah.hudson
• 0
2
votes
5
replies
1.2k
views
DESeq2 remove batch effect
BatchEffect
RNASeqData
WGCNA
DESeq2
updated 29 days ago by
Ashley
• 0 • written 11 months ago by
zxc741xb
• 0
0
votes
4
replies
628
views
How to correct for known and surrogate variables in DESeq2?
DESeq2
BatchEffect
sva
limma
updated 4 weeks ago by
Michael Love
42k • written 6 months ago by
Crystal571
• 0
0
votes
3
replies
226
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
andrebolerbarros
▴ 20
0
votes
1
reply
144
views
Deseq2 Rlog transformation
DESeq2
updated 4 weeks ago by
ATpoint
★ 4.1k • written 4 weeks ago by
Sahar
• 0
1
vote
2
replies
211
views
Design Matrix within group comparison more than two groups
DESeq2
4 weeks ago
hemantcnaik
• 0
0
votes
1
reply
149
views
Bioinformatics student
Des
DESeq2
updated 4 weeks ago by
hemantcnaik
• 0 • written 4 weeks ago by
Sahar
• 0
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Comment: Ensembl plants at annotationHub?
by
Daniele
• 0
Dear Johannes, Would it be possible to create the EnsDb for Triticum aestivum (IWGSC)? Thank you very much, Daniele
Comment: How can I create a eigencorplot of pca created from deseq2 object with PCAtools
by
BioinfGuru
▴ 30
Figured it out with ?eigencorplot, thanks Michael. I was trying to pass 10 PCs when I only had 8, and I was including metadata columns that…
Answer: DECIPHER::idTaxa - broken link to download GTDB r220 (modified) trainig set
by
makala
• 0
Thanks a lot!
Comment: DECIPHER::idTaxa - broken link to download GTDB r220 (modified) trainig set
by
Erik Wright
▴ 150
Thanks for notifying us of this broken link. It should be fixed on the Downloads page now. [1] [1]: http://www2.decipher.codes/Classificat…
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
by
Matthew Thornton
▴ 360
So it turns out that if the count and the size are the same in 'GOstats", the odds ratio is 'inf'. These are causing the issue. Thank you!!
Votes
Comment: How can I create a eigencorplot of pca created from deseq2 object with PCAtools
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Rsubread counting peaks ATACseq
Comment: Can DESeq2 rlog normalized matrix reflects time course result directly?
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