RNA-seq for DGE
1
0
Entering edit mode
amy16 • 0
@amy16-14425
Last seen 6.3 years ago

I have got RNA-seq reads from Illumina which I've trimmed adapters using trimmomatic and then aligned it with reference genome using hisat2. Now I've run through string tie for transcript identification and quantification. I would like to use ballgown for differential gene expression determination. Please advise if the pipeline I 've followed so far is right. also, I would like to use Ballgown for differential gene expression determination. Can anyone advise me on a good documentation for ballgown usage?

 

stringtie illumina hisat2 ballgown • 1.6k views
ADD COMMENT
1
Entering edit mode
@kvittingseerup-7956
Last seen 16 months ago
European Union

Your apporach sounds good although a few details are lacking to really judge it. I would suggest you take a look at stringtie's homepage where they make a nice outline including links to Ballgown usage.

ADD COMMENT

Login before adding your answer.

Traffic: 762 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6