Question: adapter trimming paired vs unpaired reads
0
gravatar for Mozart
6 months ago by
Mozart10
Mozart10 wrote:

I have been practising with Trimmomatic for days but it is still not clear to me whether or not the paired output reads (in a pair-ended sequencing) are the ones needed for the downstream alignment.

kind regards, Maria.

ADD COMMENTlink written 6 months ago by Mozart10
1

Ciao Maria, Trimmomatic is not a Bioconductor package, and is not written in R Programming Language. This support forum is primarily for Bioconductor packages.

The paired output from Trimmomatic is what you should use for alignment. For more details, I suggest posting on Biostars, which is for more general bioinformatics topics/questions.

ADD REPLYlink written 6 months ago by Kevin Blighe330

Thanks Kevin, very helpful!

ADD REPLYlink written 5 months ago by Mozart10
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 389 users visited in the last hour