Hi all. I'm working through the tutorial for single-cell analysis (https://osca.bioconductor.org/quality-control.html) and when I run this block of code
`library(scRNAseq) sce.416b <- LunSpikeInData(which="416b") sce.416b$block <- factor(sce.416b$block)`
I get the following error
`Error: failed to load resource name: AH73905 title: Ensembl 97 EnsDb for Mus musculus reason: require(“ensembldb”) failed: use BiocManager::install() to install package?`
The package installation seems to go off without an issue using the following code
but when I rerun the original code, it says that ensembldb is not installed.
Here is the output of my ensembldb installation code:
~~~~ Bioconductor version 3.11 (BiocManager 1.30.10), R 4.0.2 (2020-06-22) Installing package(s) 'ensembldb' trying URL 'https://bioconductor.org/packages/3.11/bioc/bin/macosx/contrib/4.0/ensembldb_2.12.1.tgz' Content type 'application/x-gzip' length 3403123 bytes (3.2 MB) ================================================== downloaded 3.2 MB The downloaded binary packages are in /var/folders/t7/syy6zn4d29x1ywdlr12mjg8h0000gn/T//RtmpOMOFvy/downloaded_packages ~~~
I checked the libPath() and there is only 1 path here. Does anyone have any ideas about how to solve this issue?