Dear all,
I a post-doc at the University of British Columbia, Canada and I'm pretty new to RNA-seq data analysis. I want to do the TMM normalization on my RNA-seq data using EdgeR package in R. I have two questions:
1) How can I convert .fastq files to .txt files to be able to feed them into the EdgeR package?
2) My RNA-seq data are paired sequence .fastq files. What quality control should I do on them and how should I merge them together prior to analysis?
Thanks for the help,
Hamid
According to the guideline of Rsubread, we're supposed to feed the unmapping files with txt format into the software. Is there any suggestion for the converting or can I just input the fastq files directly? Many thanks!
align
inputs FASTQ files directly. There is no suggestion in the Rsubread User's Guide or documentation that any sort of conversion is required.