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EdgeR
•
reset
0
votes
3
replies
674
views
Model performance for spline modelling of RNA-seq timecourses in edgeR
edgeR
limma
written 5 weeks ago by
skiaphrene
▴ 10
1
vote
5
replies
824
views
parameters of filterByExpr
edgeR
DESeq2
filterByExpr
updated 8 days ago by
Gordon Smyth
52k • written 11 days ago by
caroline.zanchi
• 0
10
votes
13
replies
1.8k
views
edgeR::cpm() results in negative values from raw counts of 0
edgeR
updated 20 days ago by
Gordon Smyth
52k • written 22 days ago by
Kinnary
▴ 20
2
votes
2
replies
1.2k
views
Improving performance of edgeR and limma while adjusting for multiple confounders
edgeR
limma
updated 6 weeks ago by
ATpoint
★ 4.8k • written 7 weeks ago by
robinpaul85
• 0
5
votes
3
replies
871
views
Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with multiple cell types
edgeR
limma
voomLmFit
updated 7 weeks ago by
Gordon Smyth
52k • written 7 weeks ago by
Devin
▴ 10
2
votes
2
replies
1.2k
views
Limma - Complex 2-way multi-level contrast with paired samples
edgeR
contrastmatrix
limma
9 weeks ago • updated 8 weeks ago
Elizabeth
• 0
0
votes
2
replies
493
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 8 weeks ago by
Michael Love
43k • written 9 weeks ago by
rohitsatyam102
▴ 20
0
votes
1
reply
284
views
Is it possible to model DNA methylation as a dose response in edgeR?
edgeR
updated 10 weeks ago by
Gordon Smyth
52k • written 10 weeks ago by
Isaiah
• 0
0
votes
4
replies
1.2k
views
DiffBind edgeR normalization --- why are the normalization factors all equal to 1?
edgeR
DiffBind
updated 9 weeks ago by
Harry
• 0 • written 10 weeks ago by
tifaGis
• 0
2
votes
4
replies
673
views
EdgeR Biological CVs
edgeR
written 3 months ago by
James
• 0
3
votes
5
replies
1.5k
views
Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
limma
edgeR
biomaRt
updated 3 months ago by
James W. MacDonald
68k • written 3 months ago by
Jonathan
▴ 10
0
votes
2
replies
423
views
Mean variance curve troubleshoot filtering
limmavoom
filtering
edgeR
RNASeqData
meanvariancetrend
4 months ago
gingergeiger22
• 0
0
votes
1
reply
456
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 4 months ago by
Michael Love
43k • written 4 months ago by
Jimmy Mak
• 0
1
vote
8
replies
1.2k
views
Incorporating Salmon offset matrix into Wilcoxon DEG analysis
edgeR
4 months ago
Andre
• 0
1
vote
9
replies
1.1k
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 4 months ago by
anna.cot.anna.cot
▴ 30
6
votes
10
replies
3.0k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 5 months ago by
ATpoint
★ 4.8k • written 5 months ago by
mohammedtoufiq91
▴ 10
7
votes
6
replies
1.1k
views
ANOVA like approach of edgeR
edgeR
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
Talip
▴ 10
3
votes
3
replies
708
views
Is it recommended to analyze different tissues together when using edgeR
DifferentialExpression
edgeR
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
Lars Roed
• 0
2
votes
4
replies
2.1k
views
Differential Expression Analysis with unbalanced batches without replication between conditions
edgeR
BatchEffect
updated 6 months ago by
Gordon Smyth
52k • written 6 months ago by
Thili
• 0
1
vote
8
replies
2.0k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 6 months ago by
Adam Marstrand
• 0
0
votes
1
reply
708
views
single cell RNA seq pseudobulk batch correction
edgeR
updated 6 months ago by
James W. MacDonald
68k • written 6 months ago by
mairahtkhan
• 0
3
votes
3
replies
1.2k
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 6 months ago by
Satoshi
• 0
0
votes
4
replies
822
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
6 months ago
Philippine
• 0
4
votes
6
replies
1.5k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
6 months ago
altintas.ali
• 0
1
vote
2
replies
491
views
edgeR 4.2.2 not compiled for Windows
edger
edgeR
6 months ago
Hongjian
• 0
3
votes
3
replies
499
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
7 months ago
Guido Hooiveld
★ 4.1k
4
votes
1
reply
504
views
edgeR 4.2.1 compression bug: a minimally reproducible snippet
edger
edgeR
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
Hongjian
• 0
1
vote
1
reply
397
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 7 months ago by
ATpoint
★ 4.8k • written 7 months ago by
Pimpa
• 0
1
vote
2
replies
666
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 7 months ago by
Michael Love
43k • written 7 months ago by
Ben
▴ 10
0
votes
2
replies
585
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
blmurphy
• 0
1
vote
2
replies
508
views
Inconsistent result in edgeR
edgeR
7 months ago
hiroko.ohmiya
• 0
1
vote
1
reply
667
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
erik.burchard
• 0
2
votes
9
replies
1.1k
views
Seeking Advice on Using edgeR and variancePartition for RNA-seq Data from Multiple Tissues and Patients
edgeR
variancePartition
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Seymoo
• 0
0
votes
3
replies
795
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 9 months ago by
dancingcow
• 0
1
vote
1
reply
1.7k
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData
Clustering
memory
SingleCellExperiment
edgeR
updated 9 months ago by
Aaron Lun
★ 28k • written 9 months ago by
Joseph
• 0
0
votes
1
reply
540
views
edgeR - design a complex matrix
design
model.matrix
edgeR
complexdesign
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
Karla
• 0
3
votes
3
replies
803
views
Optimizing CPM Calculation for Varied RNA-seq Data
edgeR
updated 9 months ago by
James W. MacDonald
68k • written 9 months ago by
Ahdee
▴ 60
0
votes
4
replies
824
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 10 months ago by
Shaozhen
• 0
1
vote
8
replies
1.5k
views
Advice on appropriateness of an offset in glmGamPoi/edgeR
DESeq2
glmGamPoi
offset
edgeR
scRNAseq
updated 10 months ago by
Gordon Smyth
52k • written 10 months ago by
Alec
• 0
3
votes
5
replies
2.8k
views
Right way to pseudobulk donors across replicates in edgeR
pseudobulk
edgeR
DifferentialExpression
updated 10 months ago by
Reza Ghamsari
• 0 • written 14 months ago by
Jack S.
▴ 60
0
votes
4
replies
841
views
Single Cell Experiment : Differential Expression "List of length 0" - Help!
DifferentialExpression
scran
scater
SingleCellExperiment
edgeR
11 months ago
vohara4
• 0
3
votes
6
replies
1.0k
views
Comapre two groups in edgeR- Coefficient and logFC explanation
edgeR
11 months ago
JG13
• 0
3
votes
2
replies
924
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
11 months ago
mohammedtoufiq91
▴ 10
3
votes
3
replies
984
views
How to correctly adjust for gender in edgeR
design
glm
edgeR
updated 12 months ago by
Gordon Smyth
52k • written 12 months ago by
Guillermo
• 0
1
vote
3
replies
855
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 12 months ago by
Gordon Smyth
52k • written 12 months ago by
Ashley
• 0
0
votes
1
reply
502
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 12 months ago by
Gordon Smyth
52k • written 12 months ago by
z_bahari
• 0
4
votes
2
replies
1.5k
views
Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea
edgeR
12 months ago
gene_bioconductor
▴ 10
1
vote
2
replies
1.3k
views
Citation for edgeR user guide
edgeR
written 12 months ago by
k___
▴ 30
4
votes
6
replies
1.4k
views
FilterByExpr low counts with small sample size
edgeR
13 months ago • updated 12 months ago
Jonathan
▴ 10
0
votes
2
replies
530
views
How to make DGEList and design matrix by edgeR package
DGEList
edgeR
12 months ago
prity6459
• 0
50 results • Page
1 of 1
Recent ...
Replies
Answer: DESeq2 Apeglm Coefficient Reordering / Log2FoldChange Comparison Change
by
James W. MacDonald
68k
Yes, you can simply flip the sign of the logFC to reverse the direction of the comparison.
Comment: Heatmap of a transformed deseq object with pheatmap
by
James W. MacDonald
68k
The argument for annotation_col is meant to be a `data.frame`, not a vector. The hint in the error is that it has the wrong number of dimen…
Comment: Heatmap of a transformed deseq object with pheatmap
by
caroline.zanchi
• 0
I used this argument in the second paragraph of code forthe heatmap, this is wehre I got the message Error in `[.default`(annotation_col, c…
Answer: Heatmap of a transformed deseq object with pheatmap
by
James W. MacDonald
68k
Ah, yes. The non-ASCII character error. My favorite! Please note that `pheatmap` is not a Bioconductor package, so you should be asking …
Comment: Heatmap of a transformed deseq object with pheatmap
by
caroline.zanchi
• 0
It is in ! It just took longer than planned :) ... aaaand now that I added the heatmap in I realize lits of genes really do not look like t…
Votes
Comment: Heatmap of a transformed deseq object with pheatmap
DESeq2: Question about the alpha in summary() and results()
Comment: How to load scRNA-seq data from GEO
limma correction for tumor purity
limma correction for tumor purity
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