I'm very new to the RNAseq Field. I'm trying to adjust for Sex and Age within our Dataset. I have 11 case and 12 Control samples that we have generated RNA seq data for. We've used tophat and DESEQ for the analysis. However, our samples are post mortem human brain tissue and we would want to know if there is a way to adjust for Sex and Age as each sample is either male or female and also if' it's possible to adjust for age. Does anyone know of a way to include these covariates within the analysis pipeline. Any guidance would be of great help.
Shantanu J. Shewale
Graduate School of Biomedical Sciences
Department of Molecular and Medical Genetics
University of North Texas Health Science Center